Further characterization of the yeast pso4-1 mutant: interaction with rad51 and rad52 mutants after photoinduced psoralen lesions

详细信息    查看全文

• 作者：Marcos Antonio Morais ; Elisabete José Vicente ; Jela Brozmanova ; Ana Clara Guerrini Schenberg and Jo?o Antonio Pegas Henriques
• 关键词：pso4 ; 1 mutant ; Recombinational repair ; Double ; strand breaks ; Mutagenesis ; 8 ; methoxypsoralen
• 刊名：Current Genetics
• 出版年：1996
• 出版时间：February 1996
• 年：1996
• 卷：29
• 期：3
• 页码：211-218
• 全文大小：292 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbial Genetics and Genomics
  Microbiology
  Biochemistry
  Cell Biology
  Plant Sciences
  Proteomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0983

文摘

Thepso4-1 mutant was characterized as deficient in some types of recombination, including gene conversion, crossing over, and intrachromosomal recombination. The mode of interaction betweenpso4-1 andrad51 and betweenpso4-1 andrad52 mutants indicated that thePSO4 gene belongs to theRAD52 epistasis group for strand-break repair. Moreover, the presence of thepso4-1 mutation decreased 8-MOP-photoinduced mutagenesis of therad51 andrad52 mutants. Complementation tests using heterozygous diploid strains showed that thePso4 protein might interact with theRad52 protein during repair of 8-MOP photolesions. Thepso4-1 mutant, even though defective in inter- and intea-chromosomal recombination, conserves the ability for plasmid integration of circular and linear plasmid DNA. On the other hand, similar to therad51 mutant,pso4-1 was able to incise but did not restore high-molecular-weight DNA during the repair of cross links induced by 8-MOP plus UVA. These results, together with those of previous reports, indicate that thePSO4 gene belongs to theRAD52 DNA repair group and its product participates in the DNA rejoining step of the repair of cross-link lesions, which are crucial for induced mutagenesis and recombinogenesis.