Discovery of new methylation markers to improve screening for cervical intraepithelial neoplasia grade 2/3
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- 作者：A. Boers ; R. Wang ; R. W. van Leeuwen ; H. G. Klip ; G. H. de Bock…
- 关键词：Cervical cancer screening ; Cervical precancerous lesions ; Human papillomavirus (HPV) ; Cervical scraping ; MethylCap ; seq ; DNA methylation ; Quantitative methylation ; specific PCR (QMSP)
- 刊名：Clinical Epigenetics
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：8
- 期：1
- 全文大小：830 KB
- 刊物主题：Human Genetics; Gene Function;
- 出版者：BioMed Central
- ISSN：1868-7083
文摘
Background Assessment of DNA promoter methylation markers in cervical scrapings for the detection of cervical intraepithelial neoplasia (CIN) and cervical cancer is feasible, but finding methylation markers with both high sensitivity as well as high specificity remains a challenge. In this study, we aimed to identify new methylation markers for the detection of high-grade CIN (CIN2/3 or worse, CIN2+) by using innovative genome-wide methylation analysis (MethylCap-seq). We focused on diagnostic performance of methylation markers with high sensitivity and high specificity considering any methylation level as positive.