Modifications of cell wall pectin in tomato cell suspension in response to cadmium and zinc

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• 作者：Aurélie Muschitz ; Catherine Riou ; Jean-Claude Mollet…
• 关键词：Trace elements ; Cell wall ; Pectins ; Tomato (Solanum lycopersicum L.)
• 刊名：Acta Physiologiae Plantarum
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：37
• 期：11
• 全文大小：1,594 KB
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• 作者单位：Aurélie Muschitz (1)
  Catherine Riou (1)
  Jean-Claude Mollet (2)
  Vincent Gloaguen (1)
  Céline Faugeron (1)
  
  1. Laboratoire de Chimie des Substances Naturelles, Université de Limoges, Faculté des Sciences et Techniques, 123 avenue Albert Thomas, 87060, Limoges Cedex, France
  2. Laboratoire Glycobiologie et Matrice Extracellulaire Végétale, EA4358, Normandie Université, IRIB, VASI, 76821, Mont-Saint-Aignan, France
  
• 刊物主题：Plant Physiology; Plant Genetics & Genomics; Plant Biochemistry; Plant Pathology; Plant Anatomy/Development; Agriculture;
• 出版者：Springer Berlin Heidelberg
• ISSN：1861-1664

文摘

Retention of metal cations by the cell wall is a common process found in plants in response to stress induced by the presence of trace metals (TMs). In this study conducted on a tomato cell suspension culture, cadmium (Cd) and zinc (Zn) were added to the medium at maximal concentrations of 0.5 and 2 mM, respectively. We showed that around 50 % of Zn or Cd was confined into the cell wall of tomato cells. Besides, their accumulation in the cell wall increased with the exogenous concentration in the culture medium. Characterization of cell wall pectins showed a decrease in the highly methylesterified pectin fraction whereas the weakly methylesterified pectin remained stable in response to Cd. Moreover, a significant increase in the degree of methylesterification was observed in both fractions. This was probably associated to the reduced pectin methylesterase (PME) activity in the treated cells. Furthermore, linked to a reduction of pectin content we showed a reduced expression of the galacturonosyltransferase QUA1 gene whereas PME1 expression remained unchanged. Taking together, these data strongly suggest that pectin biosynthesis and its modification in the cell wall are strongly regulated in response to TM exposure in tomato cells.