Effect of mevalonic acid on cholesterol synthesis in bovine intramuscular and subcutaneous adipocytes

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• 作者：Xiaomu Liu ; Wei You ; Haijian Cheng ; Qingfeng Zhang…
• 关键词：Mevalonic acid ; 3 ; Hydroxy ; 3 ; methylglutaryl ; CoA reductase ; Cholesterol ; Bovine adipocytes ; Meat quality
• 刊名：Journal of Applied Genetics
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：57
• 期：1
• 页码：113-118
• 全文大小：428 KB
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• 作者单位：Xiaomu Liu (1) (2)
  Wei You (1) (2)
  Haijian Cheng (1) (2)
  Qingfeng Zhang (3)
  Enliang Song (1) (2)
  Fachun Wan (1) (2)
  Hong Han (1) (2)
  Guifen Liu (1) (2)
  
  1. Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road Number 8, Ji’nan City, Shandong Province, 250100, China
  2. Shandong Key Lab of Animal Disease Control and Breeding, Jinan, 250100, China
  3. Department of Animal Science, Heze University, Heze, 274015, China
  
• 刊物主题：Life Sciences, general; Animal Genetics and Genomics; Human Genetics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
• 出版者：Springer Berlin Heidelberg
• ISSN：2190-3883

文摘

Mevalonic acid (MVA) is a key material in the synthesis of cholesterol; indeed, intracellular cholesterol synthesis is also called the mevalonic acid pathway. 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGR) is an essential enzyme in cholesterol biosynthesis. This study suggests that MVA may play an important role in the differentiation of bovine adipose tissue in vivo. We investigated differential mRNA expression in bovine intramuscular preadipocytes (BIPs) and bovine subcutaneous preadipocytes (BSPs) by culturing cells from the longissimus dorsi muscle and subcutaneous fat tissues of Luxi yellow cattle. The morphology of lipid accumulation of bovine preadipocytes was detected by Oil Red O staining, and total cholesterol (TC), low-density lipoprotein cholesterol (LDLC), and high-density lipoprotein cholesterol (HDLC) levels were measured. Temporospatial expression of HMGR was investigated by real-time quantitative polymerase chain reaction (PCR). The TC, LDLC, and HDLC content did not significantly differ over time but increased slowly with increasing MVA concentration. HMGR expression increased over time and with increasing concentrations of MVA. MVA increased adipose cell proliferation in a dose-dependent and time-dependent manner. MVA stimulated HMGR expression in two cell types and its influence on adipocyte differentiation. Keywords Mevalonic acid 3-Hydroxy-3-methylglutaryl-CoA reductase Cholesterol Bovine adipocytes Meat quality