Association of Aberrations in One Carbon Metabolism with Intimal Medial Thickening in Patients with Type 2 Diabetes Mellitus

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• 作者：R. Dhananjayan ; T. Malati ; Y. Rupasree…
• 关键词：Diabetes mellitus ; One carbon metabolism ; IMT ; Homocysteine ; Oxidative stress
• 刊名：Indian Journal of Clinical Biochemistry
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：30
• 期：3
• 页码：263-270
• 全文大小：389 KB
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• 作者单位：R. Dhananjayan (1)
  T. Malati (2)
  Y. Rupasree (3)
  Vijay Kumar Kutala (3)
  
  1. Department of Biochemistry, ACS Medical College & Hospital, Velappanchavadi, Chennai, 600 077, Tamil Nadu, India
  2. Department of Biochemistry, Nizam’s Institute of Medical Sciences, Hyderabad, 500 082, Andhra Pradesh, India
  3. Clinical Pharmacology and Therapeutics, Nizam’s Institute of Medical Sciences, Hyderabad, 500 082, Andhra Pradesh, India
  
• 刊物主题：Biochemistry, general; Microbiology; Chemistry/Food Science, general; Pathology;
• 出版者：Springer India
• ISSN：0974-0422

文摘

The present work was aimed to study the association of one carbon genetic variants, hyperhomocysteinemia and oxidative stress markers, i.e., serum nitrite, plasma malondialdehyde (MDA) and glutathione (GSH) on intimal medial thickening (IMT) in patients with type 2 diabetes mellitus (T2D). A total number of 76 subjects from ACS Medical College and Hospital, Chennai, India were included in the study, i.e., Group I (n?=?42) of T2D and Group II (n?=?34) of age- and sex matched healthy controls. The glycated haemoglobin was measured by ion-exchange resin method; plasma homocysteine by Enzyme Linked Immunosorbant Assay method; serum nitrite (nitric oxide, NO), plasma MDA and GSH by spectrophotometric methods; the IMT by high frequency ultrasound. The polymorphisms of one carbon genetic variants were genotyped using polymerase chain reaction-restriction fragment length polymorphism and amplified fragment length polymorphism methods. Results indicate that methyltetrahydrofolate homocysteine methyl transferase (MTR) A2756G allele was found to be protective in T2D and the other variants were not significantly associated with T2D. Glutamate carboxypeptidase II (GCP II) C1561T (r?=?0.34; p?=?0.05) and methylene tetrahydrofolate reductase (MTHFR) C677T (r?=?0.35; 0.04) showed positive correlation with plasma homocysteine in T2D cases. In this study, MTR A2756G allele was found to be protective in T2D; GCP II C1561T and MTHFR C677T showed positive association with plasma homocysteine in T2D cases. Among all the genetic variants, MTR A2756G was found influence IMT. RFC 1 G80A and TYMS 5-UTR 2R3R showed synergistically interact with MTR A2756G in influencing increase in IMT.