Reconstruction of the mechanisms that regulate the expression of the Escherihia coli yfiA gene under stress conditions

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• 作者：T. M. Khlebodarova (1)
  D. Yu. Oshchepkov (1)
  N. V. Tikunova (3)
  I. V. Babkin (3)
  A. D. Gruzdev (1)
  V. A. Likhoshvai (1) (2)
  
• 关键词：Escherichia coli ; yfiA ; GFP ; transcription regulation
• 刊名：Russian Journal of Genetics: Applied Research
• 出版年：2013
• 出版时间：July 2013
• 年：2013
• 卷：3
• 期：4
• 页码：271-278
• 全文大小：280KB
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• 作者单位：T. M. Khlebodarova (1)
  D. Yu. Oshchepkov (1)
  N. V. Tikunova (3)
  I. V. Babkin (3)
  A. D. Gruzdev (1)
  V. A. Likhoshvai (1) (2)
  
  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia
  3. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia
  2. Novosibirsk National Research State University, Novosibirsk, Russia
  
• ISSN：2079-0600

文摘

The regulatory region of the Escherichia coli yfiA gene was reconstructed by using the SITECON web resource and mathematical modeling; the complexity of its expression under oxidative stress was assessed. Simulation of the response of E. coli cells transformed with the pYfi-gfp plasmid to oxidative stress indicated that the maximum agreement with experimental data was achieved in a model implying complex action, apparently mediated by several transcription factors (TFs). The regulatory region of the yfiA gene was inspected for potential TF binding sites, and highly reliable predictions were made for TFs MarA, IscR, MetJ, PurR, and SoxS, which directly or indirectly participate in the response of the gene to oxidative stress, and for CRP, a global regulator of carbohydrate catabolism. The presence of binding sites for CRP, MarA, and SoxS in the E. coli yfiA promoter was confirmed by a electrophoretic mobility-shift assay with purified recombinant TFs. This fact explains the sensitivity of yfiA to mitomycin and radical-forming agents.