Molecular cloning and characterization of mevalonic acid (MVA) pathway genes and triterpene accumulation in Panax ginseng

详细信息    查看全文

• 作者：Yong-Kyoung Kim (1) (3)
  Yeon Bok Kim (1)
  Jae Kwang Kim (2)
  Soo-Un Kim (3)
  Sang Un Park (1)
  
• 关键词：gene expression ; ginsenosides ; mevalonic acid pathway ; Panax ginseng ; triterpene
• 刊名：Journal of the Korean Society for Applied Biological Chemistry
• 出版年：2014
• 出版时间：June 2014
• 年：2014
• 卷：57
• 期：3
• 页码：289-295
• 全文大小：420 KB
• 参考文献：1. Ali MB, Yu KW, Hahn EJ, and Paek KY (2005) Differential responses of anti-oxidants enzymes, lipoxygenase activity. Ascorbate content and the production of saponins in tissue cultured root of mountain / Panax ginseng C.A. Mayer and / Panax quinquefolium L. in bioreactor subjected to methyl jasmonate stress. / Plant Sci 169, 83-2. CrossRef
  2. Attele AS, Zhou YP, Xie JT, Wu JA, Zhang L, Dey L et al. (2002) Antidiabetic effects of / Panax ginseng berry extract and the identification of an effective component. / Diabetes 51, 1851-858. CrossRef
  3. Bohlmann J, Meyer-Gauen G, and Croteau R (1998) Plant terpenoid synthase: molecular biology and phylogenetic analysis. / Proc Natl Acad Sci USA 95, 4126-133. CrossRef
  4. Chen S, Luo H, Li Y, Sun Y, Wu Q, Niu Y et al. (2011) 454 EST analysis detects genes putatively involved in ginsenoside biosynthesis in / Panax ginseng. / Plant Cell Rep 30, 1593-601. CrossRef
  5. Cordier H, Karst F, and Berge’s T (1999) Heterologous expression in Saccharomyces cerevisiae of an / Arabidopsis thaliana cDNA encoding mevalonate diphosphate decarboxylase. / Plant Mol Biol 39, 953-67. CrossRef
  6. Dey L, Zhang L, and Yuan CS (2002) Anti-diabetic and anti-obese effects of ginseng berry extract: comparison between intraperitoneal and oral administrations. / Am J Chin Med 30, 645-47. CrossRef
  7. Du M and Ahn DU (2002) Simultaneous analysis of tocopherols, cholesterol, and phytosterols using gas chromatography. / J Food Sci 67, 1696-700. CrossRef
  8. Gundlach H, Mueller MJ, Kutchan TM, and Zenk MH (1992) Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. / Proc Natl Acad Sci USA 89, 2389-393. CrossRef
  9. Han JY, Kwon YS, Yang DC, Jung YR, and Choi YE (2006) Expression and RNA interference-induced silencing of the dammarenediol synthase gene in / Panax ginseng. / Plant Cell Physiol 47, 1653-662. CrossRef
  10. Hayashi H, Huang PY, and Inoue K (2003) Up-regulation of soyasaponin biosynthesis by methyl jasmonate in cultured cells of / Glycyrrhiza glabra. / Plant Cell Physio 44, 404-11. CrossRef
  11. Hu FX and Zhong JJ (2007) Role of jasmonic acid in alteration of ginsenoside heterogeneity in elicited cell cultures of / Panax notoginseng. / J Biosci Bioeng 104, 513-16. CrossRef
  12. Hu FX and Zhong JJ (2008) Jasmonic acid mediates gene transcription of ginsenoside biosynthesis in cell cultures of / Panax notoginseng treated with chemically synthesized 2-hydroxyethyl jasmonate. / Process Biochem 43, 113-18. CrossRef
  13. Hu X, Neill S, Cai W, and Tang Z (2003) Hydrogen peroxide and jasmonic acid mediate oligogalacturonic acid-induced saponin accumulation in suspension-cultured cells of / Panax ginseng. / Physiol Plant 118, 414-21. CrossRef
  14. Iishi H, Tatsuta M, Baba M, Uehara H, Nakaizumi A, Shinkai K et al. (1997) Inhibition by ginsenoside Rg3 of bombesin-enhanced peritoneal metastasis of intestinal adenocarcinomas induced by azoxymethane in Wistar rats. / Clin Exp Metast 15, 603-11. CrossRef
  15. Kim OT, Bang KH, Jung SJ, Kim YC, Hyun DY, Kim SH et al. (2010) Molecular characterization of ginseng farnesyl diphosphate synthase gene and its up-regulation by methyl jasmonate. / Biologia Plantarum 54, 47-3. CrossRef
  16. Kim SN, Ha YW, Shin HS, Son SH, Wu SJ, and Kim YS (2007) Simultaneous quantification of 14 ginsenosides in / Panax ginseng C.A. Meyer (Korean red ginseng) by HPLC-ELSD and its application to quality control. / J Pharmaceu Biomed Anal 45, 164-70. CrossRef
  17. Kim YK, Yang TJ, Kim SU, and Park SU (2012) Biochemical and molecular analysis of ginsenoside biosynthesis in / Panax ginseng during flower and berry development. / J Korean Soc Appl Biol Chem 55, 27-4. CrossRef
  18. Kim YK, Yoo DS, Xu H, Park NI, Kim HH, Choi JE et al. (2009) Ginsenoside content of berries and roots of three typical Korean ginseng ( / Panax ginseng) cultivars. / Nat Prod Commun 4, 903-06.
  19. Kubo M, Tong CN, and Matsuda H (1992) Influence of 70% methanol extract from red ginseng on the lysosome of tumor cells and on the cytocidal effect of mitomycin C. / Planta Med 58, 424-28. CrossRef
  20. Kwon SW, Han SB, Park IH, Kim JM, Park MK, and Park JH (2001) Liquid chromatographic determination of less polar ginsenosides in processed ginseng. / J Chromatogr A 921, 335-39. CrossRef
  21. Lee MH, Jeong JH, Seo JW, Shin CG, Kim YS, In JG et al. (2004) Enhanced triterpene and phytosterol biosynthesis in / Panax ginseng overexpressing squalene synthase gene. / Plant Cell Physiol 45, 976-84. CrossRef
  22. Lichtenthaler HK, Rohmer M, and Schwender J (1997a) Two independent biochemical pathway for isopentenyl diphosphate and isoprenoid biosynthesis in higher plants. / Physiol Plant 101, 643-52. CrossRef
  23. Lichtenthaler HK, Schwender J, Disch A, and Rohmer M (1997b) Biosynthesis of isoprenoids in higher plant chloroplasts proceeds via mevalonate independent pathway. / FEBS Lett 400, 271-74. CrossRef
  24. Lu MB, Wong HL, and Teng WL (2001) Effects of elicitation on the production of saponin in cell culture of / Panax ginseng. / Plant Cell Rep 20, 674-77.
  25. McGarvey DJ and Croteau R (1995) Terpenoid metabolism. / Plant Cell 7, 1015-026. CrossRef
  26. Rodriguez-Concepcion M and Boronat A (2002) Elucidation of the methylerythritol phosphate pathway for isoprenoid biosynthesis in bacteria and plastids: a metabolic milestone achieved through genomics. / Plant Physiol 130, 1079-089. CrossRef
  27. Rohmer M, Knani M, Simonin P, Sutter B, and Sahm H (1995) Isoprenoid biosynthesis in bacteria: A novel pathway for early steps leading to isopentenyl diphosphate. / Biochem J 295, 517-24.
  28. Sando T, Takaoka C, Mukai Y, Yamashita A, Hattori M, Ogasawara N et al. (2008) Cloning and characterization of mevalonate pathway genes in a natural rubber producing plant, / Hevea brasiliensis. / Biosci Biotechnol Biochem 72, 2049-060. CrossRef
  29. Schaller H, Grausem B, Benveniste P, Chye ML, Tan CT, Song YH et al. (1995) Expression of the / Hevea brasiliensis (H.B.K.) Mull. Arg. 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 in tobacco results in sterol overproduction. / Plant Physiol 109, 761-70.
  30. Shimada H, Kondo K, Fraser P, Miura Y, Saito T, and Misawa N (1998) Increased carotenoid production by the food yeast candida utilis through metabolic engineering of the isoprenoid pathway. / Appl Environ Microbiol 64, 2676-680.
  31. Shinkai K, Akedo H, Mukai M, Imamura F, Isoai A, Kobayashi M et al. (1996) Inhibition of in vitro tumor cell invasion by ginsenoside Rg3. / Jap J Cancer Res 87, 357-62. CrossRef
  32. Sticher O (1998) Getting to the root of ginseng. / Chemtech 28, 26-2.
  33. Sun C, Li Y, Wu Q, Luo H, Sun Y, Song J et al. (2010) / De novo sequencing and analysis of the American ginseng root transcriptome using a GS FLX Titanium platform to discover putative genes involved in ginsenoside biosynthesis. / BMC Genomics 11, 262. CrossRef
  34. Suzuki H, Achnine L, Xu R, Matsuda SP, and Dixon RA (2002) A genomics approach to the early stages of triterpene saponin biosynthesis in / Medicago truncatula. / Plant J 32, 1033-048. CrossRef
  35. Suzuki H, Reddy MS, Naoumkina M, Aziz N, May GD, Huhman DV et al. (2005) Methyl jasmonate and yeast elicitor induce differential transcriptional and metabolic re-programming in cell suspension cultures of the model legume / Medicago truncatula. / Planta 220, 696-70. CrossRef
  36. Yun TK (1996) Experimental and epidemiologic evidence of cancer preventive effects of / Panax ginseng C.A. Meyer. / Nutr Rev 54, S71–S81. CrossRef
  
• 作者单位：Yong-Kyoung Kim (1) (3)
  Yeon Bok Kim (1)
  Jae Kwang Kim (2)
  Soo-Un Kim (3)
  Sang Un Park (1)
  
  1. Department of Crop Science, Chungnam National University, Daejeon, 305-764, Republic of Korea
  3. Department of Agricultural Biotechnology and Research Institute for Agricultural Sciences, Seoul National University, Seoul, 151-951, Republic of Korea
  2. Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon, 406-772, Republic of Korea
  
• ISSN：2234-344X

文摘

Panax ginseng Meyer is one of the most important medicinal plants in Asia, and ginseng has attracted considerable attention worldwide. Triterpene saponins (ginsenosides) are the main bioactive compounds in P. ginseng. The isoprene units of triterpene are derived from the mevalonic acid (MVA) pathway. We cloned four genes involved in MVA pathway using rapid amplification of cDNA ends by polymerase chain reaction. Additionally, we investigated the transcript levels of 11 genes involved in the terpenoid pathway in different organs and cell suspension cultures of P. ginseng. The full-length cDNA sequences were as follows: PgHMGS (1764 bp; 1407-bp ORF), PgHMGR (1992 bp; 1722-bp ORF), PgPMK (2170 bp; 1530-bp ORF), and PgMVD (1759 bp; 1263-bp ORF). The highest expression level of all genes was found in fine roots. The total ginsenoside contents in different organs were ranked in the following descending order: leaf > fine root > lateral root > red berry > main root > petiole > stem. Campesterol and stigmasterol were detected in all organs but at different concentrations. The total phytosterol content was highest in fine root (147.8 μg/100 mg dry weight (DW)), and was lowest in the stem (86.4 μg/100 mg DW). Four enzymes in the MVA pathway were cloned and characterized in P. ginseng. Such genes play important roles in terpenoid biosynthesis and may have applications in the metabolic engineering of ginsenoside production.