Morphological features of the transgenic tobacco plant shoot expressing the 3-hydroxy-3-methylglutagyl-CoA reductase (HMG1) gene in the direct and reverse orientations towards the promoter

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• 作者：A. A. Ermoshin ; I. S. Kiseleva ; S. A. Bortsova…
• 刊名：Russian Journal of Developmental Biology
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：47
• 期：4
• 页码：216-222
• 全文大小：396 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Developmental Biology
  Animal Anatomy, Morphology and Histology
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3326
• 卷排序：47

文摘

3-Hydroxy-3-methylglutaryl-CoA reductase (HMG1) catalyzes the formation of mevalonic acid, the key intermediate of the cytosolic isoprenoid synthesis pathway. The parameters of stem and leaf growth were studied in the transgenic tobacco plants that express the HMG1 gene in both sense and antisense orientations towards the constitutive promoter. The transgenic plant height did not significantly differ from that of the control plants, though the plants carrying the sense copy of the HMG1 gene were considerably taller than plants that carried the antisense gene copy. Plants carrying an extra copy of the HMG1 gene were also characterized by increased leaf area. The number of mesophyll cells calculated per square unit of transgenic plants leaves was smaller than in the control plant leaves, though their volume was not considerably changed in any of the variants, suggesting changes in the cell packing density in leaves.Keywordstransgenic tobacco plantsHMG1 geneshoot heightleaf areamesophyll