An convenient strategy for IgG electrochemical immunosensor: the platform of topological insulator materials Bi₂Se₃ and ionic liquid

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• 作者：Sheying Dong ; Miao Li ; Wenbo Wei ; Dan Liu…
• 关键词：Electrochemical immunosensor ; Bi2Se3 ; Ionic liquid ; Human immunoglobulin G
• 刊名：Journal of Solid State Electrochemistry
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：21
• 期：3
• 页码：793-801
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Physical Chemistry; Electrochemistry; Energy Storage; Characterization and Evaluation of Materials; Analytical Chemistry; Condensed Matter Physics;
• 出版者：Springer Berlin Heidelberg
• ISSN：1433-0768
• 卷排序：21

文摘

A substantial outstanding challenge in diagnostics and disease monitoring is the ability to assay rapidly and conveniently for protein biomarkers within complex biological media. Bi₂Se₃, as an important topological insulator (TI) material, was synthesized by a solvothermal method and characterized structurally. Subsequently, the composite of Bi₂Se₃ and ionic liquid ([BMIm]BF₄ IL) was used as a sensing interface to cross-link goat anti-human immunoglobulin G (anti-IgG) via glutaraldehyde (GA) to fabricate an Bi₂Se₃/IL/GA/anti-IgG-carbon paste electrode (CPE). The nonspecific binding sites were enclosed with bovine serum albumin (BSA) to develop a label-free IgG immunosensor. The result showed that the proposed label-free IgG immunosensor exhibited high specificity with a detection limit of 0.8 ng mL−1 and linear range from 2 to 300, and 300 to 2200 ng mL−1. Besides, the immunosensor exhibited high specificity for IgG detection, acceptable reproducibility, and stability. Thus, the strategy reported here paved a simple way to design a sensitive and cost-effective sensing platform for extension to other disease biomarkers.