Mixed-strain housing for female C57BL/6, DBA/2, and BALB/c mice: validating a split-plot design that promotes refinement and reduction

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• 作者：Michael Walker ; Carole Fureix ; Rupert Palme…
• 关键词：Mice ; Refinement ; Reduction ; Animal Welfare ; Experimental Design ; Split ; Plot
• 刊名：BMC Medical Research Methodology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：16
• 期：1
• 全文大小：675 KB
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• 作者单位：Michael Walker (1)
  Carole Fureix (1)
  Rupert Palme (2)
  Jonathan A. Newman (3)
  Jamie Ahloy Dallaire (1)
  Georgia Mason (1)
  
  1. Animal Biosciences, University of Guelph, Guelph, ON, N1G 2 W1, Canada
  2. Department of Biomedical Sciences/Biochemistry, University of Veterinary Medicine, Veterinärplatz 1, Vienna, A-1210, Austria
  3. College of Biological Science, University of Guelph, Guelph, ON, N1G 2 W1, Canada
  
• 刊物主题：Theory of Medicine/Bioethics; Statistical Theory and Methods; Statistics for Life Sciences, Medicine, Health Sciences;
• 出版者：BioMed Central
• ISSN：1471-2288

文摘

Background Inefficient experimental designs are common in animal-based biomedical research, wasting resources and potentially leading to unreplicable results. Here we illustrate the intrinsic statistical power of split-plot designs, wherein three or more sub-units (e.g. individual subjects) differing in a variable of interest (e.g. genotype) share an experimental unit (e.g. a cage or litter) to which a treatment is applied (e.g. a drug, diet, or cage manipulation). We also empirically validate one example of such a design, mixing different mouse strains -- C57BL/6, DBA/2, and BALB/c -- within cages varying in degree of enrichment. As well as boosting statistical power, no other manipulations are needed for individual identification if co-housed strains are differentially pigmented, so also sparing mice from stressful marking procedures.