Biotransformation of heterocyclic dinitriles by Rhodococcus erythropolis and fungal nitrilases
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- 作者：Vojt¨§ch Vejvoda ; Ond?ej ?veda ; Ond?ej Kaplan ; V¨§ra P?ikrylov¨¢ ; Veronika Eli?¨¢kov¨¢ ; Michal Himl ; David Kub¨¢? ; Helena Pelantov¨¢ ; Marek Kuzma and Vladim¨ªr K?en ; et al.
- 关键词：Amidase ; Aspergillus niger ; Fusarium solani ; Heterocyclic dinitriles ; Nitrilase ; Nitrile hydratase ; Rhodococcus erythropolis
- 刊名：Biotechnology Letters
- 出版年：2007
- 出版时间：July, 2007
- 年：2007
- 卷：29
- 期：7
- 页码：1119-1124
- 全文大小：254 KB
文摘
2,6-Pyridinedicarbonitrile (1a) and 2,4-pyridinedicarbonitrile (2a) were hydrated by Rhodococcus erythropolis A4 to 6-cyanopyridine-2-carboxamide (1b; 83 % yield) and 2-cyanopyridine-4-carboxamide (2b; 97 % yield), respectively, after 10 min. After 118 h, the intermediates 1b or 2b were transformed into 2,6-pyridinedicarboxamide (1c; 35 % yield) and 2,6-pyridinedicarboxylic acid (1d; 60 % yield) or 2-cyanopyridine-4-carboxylic acid (2c; 64 % yield), respectively. The nitrilase from Fusarium solani afforded cyanocarboxylic acids 1e and 2c after 118 h (yields 95 and 62 % , respectively). 3,4-Pyridinedicarbonitrile (3a) and 2,3-pyrazinedicarbonitrile (4a) were inferior substrates of nitrile hydratase and nitrilase.