Microbacterium horti sp. nov., a bacterium isolated from Cucurbita maxima cultivating soil
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  • 作者:Shahina Akter ; Jae Hee Park ; Chang Shik Yin
  • 关键词:Microbacterium horti ; Gram ; reaction ; positive ; Polyphasic taxonomy ; 16S rRNA
  • 刊名:Archives of Microbiology
  • 出版年:2016
  • 出版时间:April 2016
  • 年:2016
  • 卷:198
  • 期:3
  • 页码:233-240
  • 全文大小:476 KB
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  • 作者单位:Shahina Akter (1)
    Jae Hee Park (2)
    Chang Shik Yin (3)

    1. Department of Oriental Medicinal Material and Processing College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi, 446-701, Republic of Korea
    2. Graduate School of Biotechnology, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi, 446-701, Republic of Korea
    3. Department of Acupuncture Meridian Science Research Center, College of Korean Medicine, Kyung Hee University, # 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea
  • 刊物类别:Biomedical and Life Sciences
  • 刊物主题:Life Sciences
    Microbiology
    Microbial Ecology
    Biochemistry
    Cell Biology
    Biotechnology
    Ecology
  • 出版者:Springer Berlin / Heidelberg
  • ISSN:1432-072X
文摘
A novel bacterial strain THG-SL1T was isolated from a soil sample of Cucurbita maxima garden and was characterized by using a polyphasic approach. Cells were Gram-reaction-positive, non-motile and rod-shaped. The strain was aerobic, catalase positive and weakly positive for oxidase. Phylogenetic analysis based on 16S rRNA gene sequence analysis but it shared highest similarity with Microbacterium ginsengisoli KCTC 19189T (96.6 %), indicating that strain THG-SL1T belongs to the genus Microbacterium. The DNA G + C content of the isolate was 68.9 mol %. The major fatty acids were anteiso-C15: 0 (39.7 %), anteiso-C17: 0 (24.4 %) and iso-C16: 0 (18.5 %). The major polar lipids of strain THG-SL1T were phosphatidylglycerol (PG) and an unidentified glycolipid (GL). The predominant respiratory isoprenoid quinones were menaquinone-11 and menaquinone-12. The diamino acid in the cell-wall peptidoglycan was ornithine. Based on the results of polyphasic characterization, strain THG-SL1T represented a novel species within the genus Microbacterium, for which the name Microbacterium horti sp. nov. is proposed. The type strain is THG-SL1T (=KACC 18286T=CCTCC AB 2015117T).

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