Communities of Cultivable Root Mycobionts of the Seagrass Posidonia oceanica in the Northwest Mediterranean Sea Are Dominated by a Hitherto Undescribed Pleosporalean Dark Septate Endophyte

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• 作者：Martin Vohník ; Ondřej Borovec ; Miroslav Kolařík
• 关键词：Seagrasses ; Marine fungi ; Root endophytes ; Dark septate endophytes ; Pleosporales ; Aigialaceae
• 刊名：Microbial Ecology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：71
• 期：2
• 页码：442-451
• 全文大小：6,375 KB
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• 作者单位：Martin Vohník (1) (2)
  Ondřej Borovec (1) (2)
  Miroslav Kolařík (3)
  
  1. Department of Mycorrhizal Symbioses, Institute of Botany ASCR, Lesní 322, Průhonice, 25243, Czech Republic
  2. Department of Experimental Plant Biology, Faculty of Science, Charles University in Prague, Viničná 5, Prague, 12843, Czech Republic
  3. Laboratory of Genetics, Physiology and Bioengineering of Fungi, Institute of Microbiology ASCR, Vídeňská 1083, Prague, 14200, Czech Republic
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Ecology
  Geoecology and Natural Processes
  Nature Conservation
  
• 出版者：Springer New York
• ISSN：1432-184X

文摘

Seagrasses, a small group of submerged marine macrophytes, were reported to lack mycorrhizae, i.e., the root-fungus symbioses most terrestrial plants use for nutrient uptake. On the other hand, several authors detected fungal endophytes in seagrass leaves, shoots, rhizomes, and roots, and an anatomically and morphologically unique dark septate endophytic (DSE) association has been recently described in the roots of the Mediterranean seagrass Posidonia oceanica. Nevertheless, the global diversity of seagrass mycobionts is not well understood, and it remains unclear what fungus forms the DSE association in P. oceanica roots. We isolated and determined P. oceanica root mycobionts from 11 localities in the northwest Mediterranean Sea with documented presence of the DSE association and compared our results with recent literature. The mycobiont communities were low in diversity (only three species), were dominated by a single yet unreported marine fungal species (ca. 90 % of the total 177 isolates), and lacked common terrestrial and freshwater root mycobionts. Our phylogenetic analysis suggests that the dominating species represents a new monotypic lineage within the recently described Aigialaceae family (Pleosporales, Ascomycota), probably representing a new genus. Most of its examined colonies developed from intracellular microsclerotia occupying host hypodermis and resembling microsclerotia of terrestrial DSE fungi. Biological significance of this hitherto overlooked seagrass root mycobiont remains obscure, but its presence across the NW Mediterranean Sea and apparent root intracellular lifestyle indicate an intriguing symbiotic relationship with the dominant Mediterranean seagrass. Our microscopic observations suggest that it may form the DSE association recently described in P. oceanica roots. Keywords Seagrasses Marine fungi Root endophytes Dark septate endophytes Pleosporales Aigialaceae