Sulfate-reducing bacterial communities in the water column of the Gdansk Deep (Baltic Sea)

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• 作者：V. A. Korneeva (1) (2)
  N. V. Pimenov (2)
  A. V. Krek (3)
  T. P. Tourova (1) (2)
  A. L. Bryukhanov (1) (2)
  
  1. Department of Microbiology ; Faculty of Biology ; Lomonosov Moscow State University ; Leninskie Gory 1 ; k. 12 ; Moscow ; 119234 ; Russia
  2. Winogradsky Institute of Microbiology ; Russian Academy of Sciences ; pr. 60-letiya Oktyabrya 7 ; k. 2 ; Moscow ; 117312 ; Russia
  3. Immanuel Kant Baltic Federal University ; ul. Zoologicheskaya 2 ; Kaliningrad ; 236022 ; Russia
  
• 关键词：sulfate ; reducing bacteria ; Baltic Sea ; Gdansk Deep ; chemocline ; nested PCR ; denaturing gradient gel electrophoresis (DGGE) ; dsrB gene
• 刊名：Microbiology
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：84
• 期：2
• 页码：268-277
• 全文大小：573 KB
• 参考文献：Koblents-Mishke, OI eds. (1987) Ekosistemy Baltiki v mae-iyune 1984 goda. IO AN SSSR, Moscow
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Medical Microbiology
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3237

文摘

Biodiversity of sulfate-reducing bacterial communities in the water column of the Gdansk Deep, Baltic Sea, where H2S had been detected in near-bottom layers, was analyzed by PCR with primers for the 16S rRNA genes of six major phylogenetic subgroups of sulfate-reducing bacteria (SRB). Using denaturing gradient gel electrophoresis followed by sequencing, the nucleotide sequences of reamplified dsrB gene fragments from investigated water samples were determined. For the first time the presence of nucleotide sequences of the dsrB gene was detected by PCR in the water samples from all hydrochemical layers, including subsurface oxic waters. The presence of the 16S rRNA genes of representatives of Desulfotomaculum, Desulfococcus-Desulfonema-Desulfosarcina, and Desulfovibrio-Desulfomicrobium SRB subgroups was also revealed throughout the water column of the Gdansk Deep. Analysis of translated amino acid sequences encoded by the dsrB gene demonstrated the highest homology with the relevant sequences of uncultured SRB from various marine habitats.