Towards optoelectronic urea biosensors

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• 作者：Marta Pokrzywnicka ; Robert Koncki ; ?ukasz Tymecki
• 关键词：Biosensor ; Bioreactor ; Urease ; Light ; emitting diodes ; Instrumentation ; Flow analysis
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：407
• 期：7
• 页码：1807-1812
• 全文大小：4,772 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

Integration of immobilized enzymes with light-emitting diodes (LEDs) leads to the development of optoelectronic enzyme-based biosensors. In this work, urease, used as a model enzyme, immobilized in the form of?an open-tubular microbioreactor or biosensing membrane that has been integrated with two red LEDs. It forms complete, fiberless, miniaturized, and extremely economic biooptoelectronic devices useful for nonstationary measurements under flow analysis conditions. Both enzyme-based biodevices, operating according to?the paired emitter detector diode (PEDD) principle, allow relatively fast, highly sensitive, and well-reproducible urea detection in the millimolar range of concentrations. Potential analytical applications of the developed urea bioPEDDs have been announced. Both presented constructions will be easily adapted for the development of other optoelectronic biosensors exploring various enzyme-based schemes of biodetection.