Microbioreactor designed for integration with piezoelectric transducers for cellular diagnostics
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- 作者：Yu-Hsiang Hsu (1)
William C. Tang (1) wctang@uci.edu - 关键词：Microfluidics – ; Microbioreactor – ; Cell culture – ; Starling’ ; s law – ; Cellular diagnostics
- 刊名：Microfluidics and Nanofluidics
- 出版年：2011
- 出版时间：October 2011
- 年：2011
- 卷：11
- 期：4
- 页码：459-468
- 全文大小：2.2 MB
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- 刊物类别：Engineering
- 刊物主题：Engineering Fluid Dynamics
Medical Microbiology
Polymer Sciences
Nanotechnology
Mechanics, Fluids and Thermodynamics
Engineering Thermodynamics and Transport Phenomena - 出版者：Springer Berlin / Heidelberg
- ISSN：1613-4990
文摘
This article reports a perfusion-based microbioreactor that can be integrated with a thin-film piezoelectric transducer array for rapid disease diagnosis, such as identifications of cancer cells and infection induced cell abnormality. By using the gap between suspended transducers as the high-aspect ratio barriers to establish high flow resistance into the culture chamber, we have verified that diffusion dominated the main transport mechanisms in the culture chamber. The fluid flow was successfully suppressed under 0.03, 0.3, and 3.0 μl/min volume flow rates, and a very low shear flow region was achieved on the transducer surfaces. This design offers minimal influences of mechanical forces on cellular detection and cells cultured on the surface of transducers. Finite element simulation showed that the shear stress on transducer surface could be maintained lower than milli-Pascal range. Detailed design, simulation results and experimental verifications of the microbioreactor are discussed.