Experimental evidence for the role of heterotrophic bacteria in the formation of Microcystis colonies

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• 作者：Wenjing Wang ; Hong Shen ; Pengling Shi ; Jun Chen ; Leyi Ni…
• 关键词：Microcystis ; Associated bacteria ; Colony formation ; Extracellular polysaccharides ; Metabolic compounds
• 刊名：Journal of Applied Phycology
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：28
• 期：2
• 页码：1111-1123
• 全文大小：855 KB
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• 作者单位：Wenjing Wang (1) (2)
  Hong Shen (1)
  Pengling Shi (3)
  Jun Chen (1)
  Leyi Ni (1)
  Ping Xie (1)
  
  1. Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China
  2. University of Chinese Academy of Sciences, Beijing, China
  3. College of Fisheries, Huazhong Agricultural University, Wuhan, China
  
• 刊物主题：Plant Sciences; Freshwater & Marine Ecology; Plant Physiology; Ecology;
• 出版者：Springer Netherlands
• ISSN：1573-5176

文摘

Microcystis, a genus of cyanobacteria that is dominant in eutrophic lakes, occurs mainly as colonial morphs under natural conditions but as single cells in laboratory cultures. Recent studies have suggested that Microcystis–bacteria interactions significantly influence Microcystis morphology, but the underlying mechanism remains unclear. In this study, a total of 48 strains of heterotrophic bacteria were purified from Microcystis mucilage. Five bacteria, Aeromonas veronii, Enterobacter aerogenes, Exiguobacterium acetylicum, Bacillus cereus and Shewanella putrefaciens, can induce unicellular Microcystis to form colonies. Heterotrophic bacteria stimulated Microcystis growth and induced the production of extracellular polymeric substances in coculture treatments. Extracellular polymeric substances, such as extracellular polysaccharides (EPS), were responsible for the mucilage formation in colonial Microcystis. We analysed extracellular metabolic compounds produced by Microcystis aeruginosa and Microcystis wesenbergii using gas chromatography mass spectrometry. Filtrate extracts from coculture treatments indicated that some compounds, such as 2-dodecen-1-yl(-) succinic anhydride and benzoic acid, 2,3-bis[(trimethylsilyl)oxy]-, trimethylsilyl ester, might play a significant role in colonial M. aeruginosa or M. wesenbergii formation. Our data suggested that the interaction of Microcystis and heterotrophic bacteria was crucial for the formation of Microcystis colony and outbreak of Microcystis blooms.