Celiac disease: role of intestinal compartments in the mucosal immune response

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• 作者：Giuseppe Iacomino ; Angela Marano ; Ilaria Stillitano…
• 关键词：Celiac disease ; Intestinal compartments ; Cytokines ; Innate immunity ; Adaptive immunity ; Laser capture microdissection
• 刊名：Molecular and Cellular Biochemistry
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：411
• 期：1-2
• 页码：341-349
• 全文大小：822 KB
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• 作者单位：Giuseppe Iacomino (1)
  Angela Marano (1)
  Ilaria Stillitano (1)
  Vera Rotondi Aufiero (1)
  Gaetano Iaquinto (2)
  Michele Schettino (3)
  Armando Masucci (4)
  Riccardo Troncone (5) (6)
  Salvatore Auricchio (5) (6)
  Giuseppe Mazzarella (1) (6)
  
  1. Institute of Food Sciences, CNR, via Roma 64, 83100, Avellino, Italy
  2. Gastroenterology and Digestive Endoscopy Service, S. Rita Clinic, via Appia, 83042, Atripalda, Italy
  3. Gastroenterology and Digestive Endoscopy Service, San G. Moscati Hospital, Contrada Amoretta, 83100, Avellino, Italy
  4. Department of Preventive Medicine San G. Moscati Hospital, Contrada Amoretta, 83100, Avellino, Italy
  5. Department of Translational Medicine-Pediatric Section, University of Naples “Federico II”, Naples, Italy
  6. European Laboratory for the Investigation of Food-Induced Disease (ELFID), University of Naples “Federico II”, via Pansini, 80131, Naples, Italy
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Medical Biochemistry
  Oncology
  Cardiology
  
• 出版者：Springer Netherlands
• ISSN：1573-4919

文摘

Different approaches have been used to study the pattern of cytokines in celiac disease (CD). Laser capture microdissection (LCM) is a powerful tool for the isolation of specific tissue compartments. We aimed to investigate the mucosal immune response that takes place in different intestinal compartments of CD patients, dissected by LCM, analyzing cytokine expression profile. Frozen section of jejunum was obtained from 15 untreated CD and 15 control. Surface epithelium and lamina propria compartment were isolated by LCM. RNA from each LCM sample was extracted and, after a retrotranscription step, messenger RNA levels for MxA, IL-15, TNF-α, IFN-γ, IL-17α, IL-21, IL-10, and TGF-β were determined by quantitative reverse transcriptase-PCR. Increased gene expression levels of MxA, IL-15, TNF-α, IL-10, and TGF-β was observed in the surface epithelium of untreated CD with respect to control. Furthermore, all the cytokines investigated were upregulated in the lamina propria of untreated CD as compared to control. Within the untreated CD group the expression of IL-15 was higher, in the surface epithelium than in the lamina propria, whereas the expression levels of IL-17 and IL-21 were higher in the lamina propria than in the surface epithelium. Finally, high levels of IL-10 and TGF-β were detected in both compartments of untreated CD biopsies. In CD, surface epithelium and lamina propria compartments, play a prominent role in determining innate and adaptive immunity, respectively. Conversely, surface epithelium and lamina propria produce high levels of anti-inflammatory cytokines, suggesting that both compartments are involved in the immunoregulatory response. Keywords Celiac disease Intestinal compartments Cytokines Innate immunity Adaptive immunity Laser capture microdissection