The combinatorial approach of laser-captured microdissection and reverse transcription quantitative polymerase chain reaction accurately determines HER2 status in breast cancer
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- 作者：Elisabeth Hofmann ; Rita Seeboeck ; Nico Jacobi ; Peter Obrist…
- 关键词：Breast cancer ; Personalized medicine ; HER2 ; Microdissection ; Polymerase chain reaction
- 刊名：Biomarker Research
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：4
- 期：1
- 全文大小：1,199 KB
- 刊物主题：Biomedicine general;
- 出版者：BioMed Central
- ISSN：2050-7771
文摘
Background HER2 expression in breast cancer correlates with increased metastatic potential, higher tumor recurrence rates and improved response to targeted therapies. Fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) are two methods commonly used for the analysis of HER2 in the clinic. However, lack of standardization, technical variability in laboratory protocols and subjective interpretation are major problems associated with these testing procedures.