Microfabrication of cylindrical microfluidic channel networks for microvascular research

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• 作者：Zhouchun Huang (1)
  Xiang Li (1)
  Manuela Martins-Green (2)
  Yuxin Liu (1) yuxin.liu@mail.wvu.edu
• 关键词：Reflow photoresist &#8211 ; PDMS &#8211 ; Multi ; level &#8211 ; Multi ; depth &#8211 ; Microchannels
• 刊名：Biomedical Microdevices
• 出版年：2012
• 出版时间：October 2012
• 年：2012
• 卷：14
• 期：5
• 页码：873-883
• 全文大小：680.2 KB
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• 作者单位：1. Lane Department of Computer Science and Electrical Engineering, West Virginia University, Morgantown, WV 26506, USA2. Department of Cell Biology and Neuroscience, University of California at Riverside, Riverside, CA 92521, USA
• ISSN：1572-8781

文摘

Current methods for formation of microvascular channel scaffolds are limited with non-circular channel cross-sections, complicated fabrication, and less flexibility in microchannel network design. To address current limitations in the creation of engineered microvascular channels with complex three-dimensional (3-D) geometries in the shape of microvessels, we have developed a reproducible, cost-effective, and flexible micromanufacturing process combined with photolithographic reflowable photoresist and soft lithography techniques to fabricate cylindrical microchannel and networks. A positive reflowable photoresist AZ P4620 was used to fabricate a master microchannel mold with semi-circular cross-sections. By the alignment and bonding of two polydimethylsiloxane (PDMS) microchannels replicated from the master mold together, a cylindrical microchannel or microchannel network was created. Further examination of the channel dimensions and surface profiles at different branching levels showed that the shape of the microfluidic channel was well approximated by a semi-circular surface, and a multi-level, multi-depth channel network was created. In addition, a computational fluidic dynamics (CFD) model was used to simulate shear flows and corresponding pressure distributions inside of the microchannel and channel network based on the dimensions of the fabricated channels. The fabricated multi-depth cylindrical microchannel network can provide platforms for the investigation of microvascular cells growing inside of cylindrical channels under shear flows and lumen pressures, and work as scaffolds for the investigation of morphogenesis and tubulogenesis.