Changes of Ribosomal Protein S3 Immunoreactivity and Its New Expression in Microglia in the Mice Hippocampus After Lipopolysaccharide Treatment

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• 作者：Hui Young Lee (1)
  Joon Ha Park (2)
  Choong Hyun Lee (3) vet9536@snu.ac.kr
  Bingchun Yan (2)
  Ji Hyeon Ahn (4)
  Young Joo Lee (5)
  Chan Woo Park (6)
  Jun Hwi Cho (6)
  Soo Young Choi (7)
  Moo-Ho Won (2) mhwon@kangwon.ac.kr
• 关键词：Multi ; functional protein &#8211 ; Lipopolysaccharide &#8211 ; Neuroinflammation &#8211 ; Glia
• 刊名：Cellular and Molecular Neurobiology
• 出版年：2012
• 出版时间：May 2012
• 年：2012
• 卷：32
• 期：4
• 页码：577-586
• 全文大小：1.2 MB
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• 作者单位：1. Department of Internal Medicine, Institute of Medical Sciences, School of Medicine, Kangwon National University, Chuncheon, 200-701 South Korea2. Department of Neurobiology, Institute of Medical Sciences, School of Medicine, Kangwon National University, Chuncheon, 200-701 South Korea3. Department of Anatomy and Physiology, College of Pharmacy, Dankook University, Cheonan, 330-714 South Korea4. Laboratory of Neuroscience, Department of Physical Therapy, College of Rehabilitation Science, Daegu University, Daegu, 712-714 South Korea5. Department of Emergency Medicine, Seoul Hospital, College of Medicine, Sooncheonhyang University, Seoul, 140-743 South Korea6. Department of Emergency Medicine, Institute of Medical Sciences, School of Medicine, Kangwon National University, Chuncheon, 200-701 South Korea7. Department of Biomedical Sciences, Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon, 200-702 South Korea
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Neurosciences
  Animal Anatomy, Morphology and Histology
  
• 出版者：Springer Netherlands
• ISSN：1573-6830

文摘

Lipopolysaccharide (LPS) has been used as a reagent for a model of systemic inflammatory response. Ribosomal protein S3 (rpS3) is a multi-functional protein that is involved in transcription, metastasis, DNA repair, and apoptosis. In the present study, we examined the changes of rpS3 immunoreactivity in the mouse hippocampus after systemic administration of 1 mg/kg of LPS. From 6 h after LPS treatment, rpS3 immunoreactivity was decreased in pyramidale cells of the hippocampus proper (CA1–CA3 regions) and in granule cells of the dentate gyrus. At this point in time, rpS3 immunoreactivity began to increase in non-pyramidal cells and non-granule cells. From 1 day after LPS treatment, rpS3 immunoreactivity in pyramidal and granule cells was hardly detected; however, strong rpS3 immunoreactivity was shown in non-pyramidal and non-granule cells. Based on double immunofluorescence staining for rpS3/ionized calcium-binding adapter 1 (Iba-1, a marker for microglia) and glial fibrillary acidic protein (GFAP, a marker for astrocytes), strong rpS3 immunoreactivity was expressed in Iba-1-immunoreactive microglia, not in GFAP-immunoreactive astrocytes, at 1 and 2 days after LPS treatment. These results indicate that rpS3 immunoreactivity changes only in pyramidal and granule cells, and rpS3 is expressed only in activated microglia after LPS treatment: this may be associated with the neuroinflammatory responses in the brain.