Changes in Phenolics Distribution After Chemical Pretreatment and Enzymatic Conversion of Miscanthus 脳 giganteus Internode

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• 作者：N. Belmokhtar (1) (2) (3)
  A. Habrant (1) (2)
  N. Lopes Ferreira (3)
  B. Chabbert (1) (2)
  
• 关键词：Lignin ; Hydroxycinnamic acids ; Saccharification ; UV microspectrophotometry
• 刊名：Bioenergy Research
• 出版年：2013
• 出版时间：June 2013
• 年：2013
• 卷：6
• 期：2
• 页码：506-518
• 全文大小：810KB
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• 作者单位：N. Belmokhtar (1) (2) (3)
  A. Habrant (1) (2)
  N. Lopes Ferreira (3)
  B. Chabbert (1) (2)
  
  1. INRA, UMR614 Fractionnement des AgroRessources et Environnement, 51100, Reims Cedex, France
  2. Universit茅 de Reims Champagne-Ardenne, UMR614 Fractionnement des AgroRessources et Environnement, 51100, Reims, France
  3. IFP Energies nouvelles, 1&4 avenue du Bois Pr茅au, Rueil-Malmaison, 92500, France
  
• ISSN：1939-1242

文摘

In addition to lignin, grass cell walls are characterized by the presence of hydroxycinnamic acids that play a significant role in cross-linking polymers into a cohesive network, and pretreatments are required to overcome the recalcitrance of lignocelluloses prior to enzymatic bioconversion of polysaccharides. The effects of dilute acid and ammonium hydroxide pretreatments were studied on the chemical composition and enzymatic saccharification of Miscanthus internodes fragments. The hydroxycinnamic acid content was reduced after both pretreatments, while lignin got enriched in condensed linked structures. In addition, dilute acid pretreatment was effective in decreasing xylan content of Miscanthus, while ammonia treatment induced a marked swelling effect on the cell walls of parenchyma, vascular sclerenchyma, and epidermal sclerenchyma. The phenol distribution at the cell level was estimated using UV transmission microspectrophotometry. Internode cell walls displayed different UV spectra according to the cell type. However, the secondary cell walls had similar UV spectra after pretreatment, whereas spectra recorded at the cell corner region displayed variations according to cell type and pretreatment. Acid pretreatment was more efficient than ammonia to improve the conversion of polysaccharides by a Trichoderma cellulolytic cocktail. Although pretreatments achieved moderate saccharification yields, the secondary cell walls were altered at some pit regions of the vascular sclerenchyma whereas parenchyma appeared recalcitrant. Variations in the UV spectra of enzyme-digested cell walls suggest pretreatment-dependent heterogeneity of the phenolic distribution in the more recalcitrant cell walls.