Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry

详细信息    查看全文

• 作者：Zuzana Burdikova ; Zdenek Svindrych ; Cian Hickey…
• 关键词：Confocal microscopy ; Cheese matrix ; Fluorescence lifetime ; Second harmonic generation ; Two ; photon excitation ; Confocal Raman microscopy
• 刊名：Dairy Science & Technology
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：95
• 期：5
• 页码：687-700
• 全文大小：1,730 KB
• 参考文献：Auty MAE, Gardiner GE, McBrearty SJ et al (2001) Direct in situ viability assessment of bacteria in probiotic dairy products using viability staining in conjunction with confocal scanning laser microscopy. Appl Environ Microbiol 67:420鈥?25. doi:10.鈥?128/鈥婣EM.鈥?7.鈥?.鈥?20-425.鈥?001 CrossRef
  Banks W (1991) Milk lipids. Bull Int Dairy Fed 260:3鈥?
  Bernatov谩 S, Samek O, Pil谩t Z et al (2013) Following the mechanisms of bacteriostatic versus bactericidal action using Raman spectroscopy. Molecules 18:13188鈥?3199. doi:10.鈥?390/鈥媘olecules1811131鈥?8 CrossRef
  Bianchini P, Diaspro A (2008) Three-dimensional (3D) backward and forward second harmonic generation (SHG) microscopy of biological tissues. J Biophotonics 1:443鈥?50. doi:10.鈥?002/鈥媕bio.鈥?00810060 CrossRef
  Burd铆kov谩 Z, 膶apek M, Osta拧ov P et al (2010) Testate amoebae examined by confocal and two-photon microscopy: implications for taxonomy and ecophysiology. Microsc Microanal 16:735鈥?46CrossRef
  Burdikova Z, Svindrych Z, Pala J et al (2015) Measurement of pH micro-heterogeneity in natural cheese matrices by fluorescence lifetime imaging. Front Microbiol. doi:10.鈥?389/鈥媐micb.鈥?015.鈥?0183
  Daly DFM, McSweeney PLH, Sheehan JJ (2012) Pink discolouration defect in commercial cheese: a review. Dairy Sci Technol 92:439鈥?53. doi:10.鈥?007/鈥媠13594-012-0079-0 CrossRef
  De Gelder J, De Gussem K, Vandenabeele P, Moens L (2007) Reference database of Raman spectra of biological molecules. J Raman Spectrosc 38:1133鈥?147. doi:10.鈥?002/鈥媕rs.鈥?734 CrossRef
  Diaspro A, Fronte P, Raimondo M, et al. (2002) Functional imaging of living Paramecium by means of confocal and two-photon excitation fluorescence microscopy. pp 24鈥?1
  Diaspro A, Bianchini P, Vicidomini G et al (2006) Multi-photon excitation microscopy. Biomed Eng Online 5:36. doi:10.鈥?186/鈥?475-925X-5-36 CrossRef
  El-Bakry M, Sheehan J (2014) Analysing cheese microstructure: a review of recent developments. J Food Eng 125:84鈥?6. doi:10.鈥?016/鈥媕.鈥媕foodeng.鈥?013.鈥?0.鈥?30 CrossRef
  Everett DW, Auty MAE (2008) Cheese structure and current methods of analysis. Int Dairy J 18:759鈥?73. doi:10.鈥?016/鈥媕.鈥媔dairyj.鈥?008.鈥?3.鈥?12 CrossRef
  Filov谩 E, Burd铆kov谩 Z, Rampichov谩 M et al (2010) Analysis and three-dimensional visualization of collagen in artificial scaffolds using nonlinear microscopy techniques. J Biomed Opt 15:66011鈥?6017CrossRef
  Gallier S, Gragson D, Jim茅nez-Flores R, Everett D (2010) Using confocal laser scanning microscopy to probe the milk fat globule membrane and associated proteins. J Agric Food Chem 58:4250鈥?257. doi:10.鈥?021/鈥媕f9032409 CrossRef
  Harz M, Rosch P, Peschke K-D et al (2005) Micro-Raman spectroscopic identification of bacterial cells of the genus Staphylococcus and dependence on their cultivation conditions. Analyst 130:1543鈥?550. doi:10.鈥?039/鈥婤507715J CrossRef
  Haupert LM, DeWalt EL, Simpson GJ (2012) Modeling the SHG activities of diverse protein crystals. Acta Crystallogr Sect D: Biol Crystallogr 68:1513鈥?521. doi:10.鈥?107/鈥婼090744491203763鈥? CrossRef
  Hickey CD, Sheehan JJ, Wilkinson MG, Auty MAE (2015) Growth and location of bacterial colonies within dairy foods using microscopy techniques: a review. Front Microbiol. doi:10.鈥?389/鈥媐micb.鈥?015.鈥?0099
  Hollricher O, Ibach W (2011) Confocal Raman microscopy. Springer, Berlin
  Jeanson S, Chad艙uf J, Madec MN et al (2011) Spatial distribution of bacterial colonies in a model cheese. Appl Environ Microbiol 77:1493鈥?00. doi:10.鈥?128/鈥婣EM.鈥?2233-10 CrossRef
  Jose J, Burgess K (2006) Benzophenoxazine-based fluorescent dyes for labeling biomolecules. Tetrahedron 62:11021鈥?1037. doi:10.鈥?016/鈥媕.鈥媡et.鈥?006.鈥?8.鈥?56 CrossRef
  Kalab M (1995) Practical aspects of electron microscopy in cheese research. In: Malin EL, Tunick MH (eds) Chemistry of structure-function relationships in cheese. Plenum Press, New York, pp 247鈥?76CrossRef
  Kondo Y, Watanabe T, Aita S (1990) Application of the frozen thin sectioning method to the observation and X-ray microanalysis of cheese using an analytical transmission electron microscope. J Jpn Soc Food Sci Technol 37:45鈥?7CrossRef
  Lakowicz JR (2006) Principles of fluorescence spectroscopy, 3rd ednCrossRef
  Laloy E, Vuillemard JC, El Soda ME, Simard RE (1996) Influence of the fat content of Cheddar cheese on retention and localization of starters. Int Dairy J 6:729鈥?40. doi:10.鈥?016/鈥?958-6946(95)00068-2 CrossRef
  Lawrence RC, Gilles J, Creamer LK et al (2004) Cheddar cheese and related dry-salted cheese varieties, 3rd edn. Elsevier Academic Press, London
  Lopez C, Briard-Bion V (2007) The composition, supramolecular organisation and thermal properties of milk fat: a new challenge for the quality of food products. Lait 87:317鈥?36CrossRef
  Lopez C, Maillard MB, Briard-Bion V et al (2006) Lipolysis during ripening of Emmental cheese considering organization of fat and preferential localization of bacteria. J Agric Food Chem 54:5855鈥?867. doi:10.鈥?021/鈥媕f060214l CrossRef
  Lopez C, Camier B, Gassi J-Y (2007) Development of the milk fat microstructure during the manufacture and ripening of Emmental cheese observed by confocal laser scanning microscopy. Int Dairy J 17:235鈥?47. doi:10.鈥?016/鈥媕.鈥媔dairyj.鈥?005.鈥?2.鈥?15 CrossRef
  Lopez C, Briard-Bion V, Beaucher E, Ollivon M (2008) Multiscale characterization of the organization of triglycerides and phospholipids in Emmental cheese: from the microscopic to the molecular level. J Agric Food Chem 56:2406鈥?414. doi:10.鈥?021/鈥媕f0720382 CrossRef
  Lucey JA, Fox PF (2015) Importance of calcium and phosphate in cheese manufacture: a review. J Dairy Sci 76:1714鈥?724. doi:10.鈥?168/鈥媕ds.鈥婼0022-0302(93)77504-9 CrossRef
  Maquelin K, Kirschner C, Choo-Smith LP et al (2002) Identification of medically relevant microorganisms by vibrational spectroscopy. J Microbiol Methods 51:255鈥?71CrossRef
  Movasaghi Z, Rehman S, Rehman IU (2007) Raman spectroscopy of biological tissues. Appl Spectrosc Rev 42:493鈥?41CrossRef
  Notingher I (2007) Raman spectroscopy cell-based biosensors. Sensors 7:1343鈥?358CrossRef
  Pawley JB (2006) Handbook of biological confocal microscopy, 3rd edn. Springer Science & Business Media, New YorkCrossRef
  Pearce KN, Creamer LK, Gilles J (1973) Calcium lactate deposits on rindless cheddar cheese. N Z J Dairy Sci Technol 8:3鈥?
  Pereira CI, Gomes AMP, Xavier Malcata F (2009) Microstructure of cheese: processing, technological and microbiological considerations. Trends Food Sci Technol 20:213鈥?19. doi:10.鈥?016/鈥媕.鈥媡ifs.鈥?009.鈥?2.鈥?06 CrossRef
  Samek O, Al-Marashi JFM, Telle HH (2010) The potential of Raman spectroscopy for the identification of biofilm formation by Staphylococcus epidermidis. Laser Phys Lett 7:378鈥?83. doi:10.鈥?002/鈥媗apl.鈥?00910154 CrossRef
  Samek O, Mlynarikov谩 K, Bernatov谩 S et al (2014) Candida parapsilosis biofilm identification by Raman spectroscopy. Int J Mol Sci 15:23924鈥?3935. doi:10.鈥?390/鈥媔jms151223924 CrossRef
  Scott R (1981) Cheesemaking practice. Elsevier Applied Science Publishers, London
  艩epitka J, Luke拧 J, Stan臎k L et al (2012) Nanoindentation of intervertebral disc tissues localised by SHG imaging. Comput Methods Biomech Biomed Eng 15:335鈥?36. doi:10.鈥?080/鈥?0255842.鈥?012.鈥?13601 CrossRef
  Sheehan JJ, Guinee TP (2004) Effect of pH and calcium level on the biochemical, textural and functional properties of reduced-fat Mozzarella cheese. Int Dairy J 14:161鈥?72CrossRef
  Shinzawa H, Awa K, Kanematsu W, Ozaki Y (2009) Multivariate data analysis for Raman spectroscopic imaging. J Raman Spectrosc 40:1720鈥?725. doi:10.鈥?002/鈥媕rs.鈥?525 CrossRef
  Upreti P, Metzger LE (2006) Influence of calcium and phosphorus, lactose, and salt-to-moisture ratio on cheddar cheese quality: manufacture and composition. J Dairy Sci 89:420鈥?28. doi:10.鈥?168/鈥媕ds.鈥婼0022-0302(06)72106-3 CrossRef
  Washam CJ, Kerr TJ, Hurst VJ, Rigsby WE (1985) A scanning electron microscopy study of crystalline structures on commercial cheese. Dev Ind Microbiol 26:749鈥?61
  Yiu SH (1985) A fluorescence microscopic study of cheese. Food Microstruct 4:99鈥?06
  
• 作者单位：Zuzana Burdikova (1)
  Zdenek Svindrych (2)
  Cian Hickey (1)
  Martin G. Wilkinson (4)
  Mark A. E. Auty (1)
  Ota Samek (3)
  Silvia Bernatova (3)
  Vladislav Krzyzanek (3)
  Ammasi Periasamy (2)
  Jeremiah J. Sheehan (1)
  
  1. Teagasc Food Research Centre, Moorepark, Fermoy, Co., Cork, Ireland
  2. Department of Biology, University of Virginia, Charlottesville, VA, USA
  4. Department of Life Sciences, University of Limerick, Limerick, Ireland
  3. VUT, Brno, Czech Republic
  
• 刊物主题：Food Science; Agriculture; Microbiology;
• 出版者：Springer Paris
• ISSN：1958-5594

文摘

Despite considerable advances in confocal microscopy and related technologies, advanced light microscopy techniques have been rarely used to study the microstructure of cheese matrices. Here, we demonstrate the potential of advanced microscopic techniques, namely fluorescence lifetime imaging (FLIM), confocal Raman spectroscopy and non-linear microscopy (two-photon excitation and second harmonic generation microscopy), to study localized physicochemical properties of cheese matrices. These methods allow for precise localization of individual chemical components and help to determine their spatial organization in three dimensions. We discuss both fluorescent labelling and label-free methods that provide valuable information about the localized environment. Overall, these new technologies will enable a greater understanding of the influence of manufacturing processes on cheese quality and consistency. Keywords Confocal microscopy Cheese matrix Fluorescence lifetime Second harmonic generation Two-photon excitation Confocal Raman microscopy