Exploring the effects of cell seeding density on the differentiation of human pluripotent stem cells to brain microvascular endothelial cells
详细信息 查看全文
- 作者：Hannah K Wilson ; Scott G Canfield ; Michael K Hjortness…
- 关键词：In vitro human blood–brain barrier (BBB) model ; Human pluripotent stem cells (hPSCs) ; Brain microvascular endothelial cells ; Transendothelial electrical resistance ; Efflux transporters ; Tight junctions ; Seeding density
- 刊名：Fluids and Barriers of the CNS
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：12
- 期：1
- 全文大小：8,064 KB
- 刊物主题：Neurosciences; Neurology;
- 出版者：BioMed Central
- ISSN：2045-8118
文摘
Background Brain microvascular-like endothelial cells (BMECs) derived from human pluripotent stem cells (hPSCs) have significant promise as tools for drug screening and studying the structure and function of the BBB in health and disease. The density of hPSCs is a key factor in regulating cell fate and yield during differentiation. Prior reports of hPSC differentiation to BMECs have seeded hPSCs in aggregates, leading to non-uniform cell densities that may result in differentiation heterogeneity. Here we report a singularized-cell seeding approach compatible with hPSC-derived BMEC differentiation protocols and evaluate the effects of initial hPSC seeding density on the subsequent differentiation, yield, and blood–brain barrier (BBB) phenotype.