Brain-Derived Neurotrophic Factor Inhibits Intercellular Adhesion Molecule-1 Expression in Interleukin-1β-Treated Endothelial Cells

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• 作者：Katsuhiro Takeda ; Yusuke Obinata ; Akihiro Konishi…
• 关键词：Brain ; derived neurotrophic factor ; Interleukin ; 1β ; Vascular endothelial cells ; Periodontal therapy
• 刊名：Cell Biochemistry and Biophysics
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：74
• 期：3
• 页码：399-406
• 全文大小：6,376 KB
• 刊物主题：Biochemistry, general; Pharmacology/Toxicology; Biotechnology; Cell Biology; Biophysics and Biological Physics;
• 出版者：Springer US
• ISSN：1559-0283
• 卷排序：74

文摘

Brain-derived neurotrophic factor (BDNF) enhances periodontal tissue regeneration. Tissue regeneration is characterized by inflammation, which directs the quality of tissue repair. The objective of this study is to propose the relevance of BDNF to inflammation. In this study, we investigated the effect of BDNF on intercellular adhesion molecule (ICAM)-1, which is an inflammatory marker, expressed in interleukin (IL)-1β-treated human microvascular endothelial cells (HMVECs). In addition, we studied the effect of BDNF on the adhesion of neutrophil-like differentiated HL-60 cells to HMVECs in a cell adhesion assay. We demonstrated that BDNF attenuates the IL-1β-induced ICAM-1 mRNA and protein expression. Treatment of HMVECs with IL-1β led to an increase in the number of adherent neutrophil-like HL-60 cells. BDNF significantly decreased the number of neutrophil-like HL-60 cells attached to HMVECs. In conclusion, BDNF may reduce excess inflammation through reduced neutrophil recruitment by regulating ICAM-1 expression.