Ectopic Hard Tissue Formation by Odonto/Osteogenically In Vitro Differentiated Human Deciduous Teeth Pulp Stem Cells

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• 作者：Seunghye Kim ; Je Seon Song ; Mijeong Jeon ; Dong Min Shin…
• 关键词：Deciduous teeth pulp stem cell ; Odonto/osteogenic in vitro differentiation ; Hard tissue
• 刊名：Calcified Tissue International
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：97
• 期：1
• 页码：80-89
• 全文大小：7,876 KB
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• 作者单位：Seunghye Kim (1) (2)
  Je Seon Song (1) (2)
  Mijeong Jeon (2)
  Dong Min Shin (3)
  Seong-Oh Kim (1) (2)
  Jae Ho Lee (1) (2)
  
  1. Department of Pediatric Dentistry, College of Dentistry, Yonsei University, 250 Seongsanno, Seodaemun-gu, Seoul, 120-752, Korea
  2. Oral Science Research Center, College of Dentistry, Yonsei University, Seoul, Korea
  3. Department of Oral Biology, College of Dentistry, Yonsei University, Seoul, Korea
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Endocrinology
  Orthopedics
  Cell Biology
  
• 出版者：Springer New York
• ISSN：1432-0827

文摘

There have been many attempts to use the pulp tissue from human deciduous teeth for dentin or bone regeneration. The objective of this study was to determine the effects of odonto/osteogenic in vitro differentiation of deciduous teeth pulp stem cells (DTSCs) on their in vivo hard tissue-forming potential. DTSCs were isolated from extracted deciduous teeth using the outgrowth method. These cells were exposed to odonto/osteogenic stimuli for 4 and 8?days (Day 4 and Day 8 groups, respectively), while cells in the control group were cultured in normal medium. The in vitro differentiated DTSCs and the control DTSCs were transplanted subcutaneously into immunocompromised mice with macroporous biphasic calcium phosphate and sacrificed at 8?weeks post-implantation. The effect of odonto/osteogenic in vitro differentiation was evaluated using alkaline phosphatase (ALP) staining and quantitative reverse transcription polymerase chain reaction (RT-PCR). The in vivo effect was evaluated by qualitative RT-PCR, assessment of ALP activity, histologic analysis, and immunohistochemical staining. The amount of hard tissue was greater in Day 4 group than Day 8 group (p?=?0.014). However, Day 8 group generated lamellar bone-like structure, which was immunonegative to anti-human dentin sialoprotein with significantly low expression level of DSPP compared with the control group (p?=?0.008). This study demonstrates that odonto/osteogenic in vitro differentiation of DTSCs enhances the formation of bone-like tissue, instead of dentin-like tissue, when transplanted subcutaneously using MBCP as a carrier. The odonto/osteogenic in vitro differentiation of DTSCs may be an effective modification that enhances in vivo bone formation by DTSCs.