Polymorphism analysis of tyrosine hydroxylase (TH) in military working dogs

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• 作者：Jungwoo Eo (1)
  Bong-Hwan Choi (2)
  Yi-Deun Jung (1)
  Yun-Jeong Kwon (1)
  Tae-Hun Kim (2)
  Hwan-Hoo Seong (2)
  Heui-Soo Kim (1)
  
• 关键词：Military working dogs ; Polymorphism ; Tyrosine hydroxylase ; Variable number of tandem repeats
• 刊名：Genes & Genomics
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：35
• 期：6
• 页码：817-821
• 全文大小：
• 作者单位：Jungwoo Eo (1)
  Bong-Hwan Choi (2)
  Yi-Deun Jung (1)
  Yun-Jeong Kwon (1)
  Tae-Hun Kim (2)
  Hwan-Hoo Seong (2)
  Heui-Soo Kim (1)
  
  1. Department of Biological Sciences, College of Natural Sciences, Pusan National University, Pusan, 609-735, Republic of Korea
  2. Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration, Suwon, 441-706, Republic of Korea
  
• ISSN：2092-9293

文摘

Canine and human behavior are shaped by similar evolutionary processes, yet the identification of the behavioral phenotype is often difficult. A widely used method relies on breed stereotypes provided by experts such as dog trainers. To reveal a valid association between behavior and genetic factors, an association study of behavioral phenotyping and genotyping is essential. We screened for variable number of tandem repeat (VNTR) polymorphisms in intron 4 of the tyrosine hydroxylase gene in military working dogs (belonging to pass and fail groups based on the results of an in-training examination conducted by the drillmaster), which were scored based on possessiveness, audaciousness, concentration, and motor ability by qualification examination. We first characterized each genotype by sequencing, in which the 1/1 type consists of a single copy of a 36-bp sequence and the 2/2 type is a duplicated form of the 36-bp repeat unit. The 1/2 alleles showed a single nucleotide change as a heteroduplex, which generated a PCR product of similar size as that of the 1/1-182-bp. The military working dogs showed the 2/2 type of VNTR and heteroduplex. For the pass group, two dogs possessed 2/2 type (40?%), whereas three dogs were of the heteroduplex type (60?%). However, all members of fail group showed the 2/2 type (100?%). These data indicate that repeat polymorphisms with behavioral phenotyping can identify military working dogs that would pass or fail the in-training examination.