Practical fabrication of microfluidic platforms for live-cell microscopy

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• 作者：Daniel Lorusso ; Hristo N. Nikolov ; Jaques S. Milner…
• 刊名：Biomedical Microdevices
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：18
• 期：5
• 全文大小：1,832 KB
• 刊物类别：Engineering
• 刊物主题：Biomedical Engineering
  Biophysics and Biomedical Physics
  Nanotechnology
  Engineering Fluid Dynamics
  
• 出版者：Springer Netherlands
• ISSN：1572-8781
• 卷排序：18

文摘

We describe a simple fabrication technique – targeted towards non-specialists – that allows for the production of leak-proof polydimethylsiloxane (PDMS) microfluidic devices that are compatible with live-cell microscopy. Thin PDMS base membranes were spin-coated onto a glass-bottom cell culture dish and then partially cured via microwave irradiation. PDMS chips were generated using a replica molding technique, and then sealed to the PDMS base membrane by microwave irradiation. Once a mold was generated, devices could be rapidly fabricated within hours. Fibronectin pre-treatment of the PDMS improved cell attachment. Coupling the device to programmable pumps allowed application of precise fluid flow rates through the channels. The transparency and minimal thickness of the device enabled compatibility with inverted light microscopy techniques (e.g. phase-contrast, fluorescence imaging, etc.). The key benefits of this technique are the use of standard laboratory equipment during fabrication and ease of implementation, helping to extend applications in live-cell microfluidics for scientists outside the engineering and core microdevice communities.KeywordsFluid shear stressMicroscopyComputational fluid dynamicsMicro-particle image velocimetryPDMSMicrowaveMicrofluidics