Effects of Fluoride on Expression of P450, CREM and ACT Proteins in Rat Testes

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• 作者：Jianhai Zhang ; Yuchen Zhu ; Chen Liang ; Mingli Qie&#8230
• 关键词：Fluoride ; P450 ; CREM ; ACT ; Testes
• 刊名：Biological Trace Element Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：175
• 期：1
• 页码：156-160
• 全文大小：
• 刊物主题：Biochemistry, general; Biotechnology; Nutrition; Oncology;
• 出版者：Springer US
• ISSN：1559-0720
• 卷排序：175

文摘

Fluoride (F) is an essential trace element that humans and animals ingest from water, air, and fluoride-containing products; however, excessive fluoride absorption can damage a variety of organs and tissues, including the male reproductive system. Our previous studies found that fluoride exposure lowered sperm quality and interfered with spermatogenesis; however, the exact mechanism remained unclear. Proteins cytochrome P450 (P450), cAMP-responsive element modulator (CREM), and activator of CREM in testis (ACT) play the key roles in spermatogenesis and sperm motility. To investigate whether fluoride affects the expression of P450, CREM, and ACT, we used immunohistochemical techniques to determine expression levels of these proteins in testes of rats administered 100 mg NaF/L for 2 weeks via drinking water. The results showed that P450 expression was decreased while CREM and ACT expression was increased in the fluoride group, compared to the control. These data suggest that fluoride can impair male reproduction by affecting expression of P450, CREM, and ACT in the testes.