Ethyl acetate extract of Wedelia chinensis inhibits tert-butyl hydroperoxide-induced damage in PC12 cells and D-galactose-induced neuronal cell loss in mice

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• 作者：Wea-Lung Lin (25) (26)
  Shao-Ming Wang (27)
  Ying-Jui Ho (28)
  Hsing-Chun Kuo (29)
  Yean-Jang Lee (30)
  Tsui-Hwa Tseng (27) (31)
  
  25. Department of Pathology ; Chung Shan Medical University Hospital ; No. 110 ; Section 1 ; Chien-Kuo N. Road ; Taichung ; 402 ; Taiwan
  26. School of Medicine ; Chung Shan Medical University ; No. 110 ; Section 1 ; Chien-Kuo N. Road ; Taichung ; Taiwan
  27. School of Medical Applied Chemistry ; Chung Shan Medical University ; No. 110 ; Section 1 ; Chien-Kuo N. Road ; Taichung ; 402 ; Taiwan
  28. School of Psychology ; Chung Shan Medical University ; No. 110 ; Section 1 ; Chien-Kuo N. Road ; Taichung ; 402 ; Taiwan
  29. Department of Nursing ; Chang Gung University of Science and Technology ; Chia-Yi Campus No. 2 ; Chia-Pu Rd. West Sec. Putz ; Chia-Yi ; 613 ; Taiwan
  30. Department of Chemistry ; National Changhua University of Education ; No. 1 ; Jin-De Road ; Changhua ; 500 ; Taiwan
  31. Department of Medical Education ; Chung Shan Medical University Hospital ; No. 110 ; Section 1 ; Chien-Kuo N. Road ; Taichung ; 402 ; Taiwan
  
• 关键词：Apoptosis ; t ; butylhydroperoxide ; D ; galactose ; Luteolin ; Wedelia chinensis ; Wedelolactone
• 刊名：BMC Complementary and Alternative Medicine
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：14
• 期：1
• 全文大小：1,933 KB
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  33. The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/14/491/prepub
  
• 刊物主题：Complementary & Alternative Medicine; Internal Medicine; Chiropractic Medicine;
• 出版者：BioMed Central
• ISSN：1472-6882

文摘

Background Wedelia chinensis is traditionally used as a hepatoprotective herb in Taiwan. The aim of this study was to evaluate the neuroprotective potential of W. chinensis. Methods An ethyl acetate extract of W. chinensis (EAW) was prepared and analyzed by HPLC. The neuroprotective potential of EAW was assessed by tert-butylhydroperoxide (t-BHP)-induced damage in PC12 cells and D-galactose-induced damage in mouse cortex. Results EAW exhibited potent radical scavenging property and highly contained luteolin and wedelolactone. EAW decreased t-BHP-induced reactive oxygen species (ROS) accumulation, cytotoxicity and apoptosis in PC12 cells. EAW and its major constituents blocked t-BHP-induced cytochrome C release and Bcl-2 family protein ratio change. EAW and its major constituents increased the endogenous antioxidant capacity evaluated by the binding activity assay of nuclear factor E2-related factor 2 (Nrf2) to antioxidant response element (ARE) and nuclear translocation of Nrf2 respectively in PC12 cells. Finally, EAW inhibited D-galactose-induced lipid peroxidation, apoptosis and neuron loss in the cerebral cortex of mice. Conclusion These results demonstrate that W. chinensis has neuroprotective potential through blocking oxidative stress-induced damage and that luteolin and wedelolactone contribute to the protective action.