Solid freeform fabrication and in-vitro response of osteoblast cells of mPEG-PCL-mPEG bone scaffolds

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• 作者：Cho-Pei Jiang (1)
  Yo-Yu Chen (2)
  Ming-Fa Hsieh (3)
  Hung-Maan Lee (4)
  
• 关键词：Poly(ethylene glycol) ; Poly(ε ; caprolactone) ; Scaffold ; Osteoblast ; Solid freeform fabrication
• 刊名：Biomedical Microdevices
• 出版年：2013
• 出版时间：April 2013
• 年：2013
• 卷：15
• 期：2
• 页码：369-379
• 全文大小：731KB
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• 作者单位：Cho-Pei Jiang (1)
  Yo-Yu Chen (2)
  Ming-Fa Hsieh (3)
  Hung-Maan Lee (4)
  
  1. Department of Power Mechanical Engineering, National Formosa University, Yunlin County, Taiwan
  2. Institute of Mechanical and Electro-Mechanical Engineering, National Formosa University, Yunlin County, Taiwan
  3. Department of Biomedical Engineering, Chung Yuan Christian University, Chung Li, Taiwan
  4. Hualien Armed Forces General Hospital, Hualien, Taiwan
  
• ISSN：1572-8781

文摘

Bone tissue engineering is an emerging approach to provide viable substitutes for bone regeneration. Poly(ethylene glycol) (PEG) is a good candidate of bone scaffold because of several advantages such as hydrophilicity, biocompatibility, and intrinsic resistance to protein adsorption and cell adhesion. However, its low compressive strength limits application for bone regeneration. Poly(ε-caprolactone) (PCL), a hydrophobic nonionic polymer, is adopted to enhance the compressive strength of PEG alone.We aimed to investigate the in-vitro response of osteoblast-like cells cultured with porous scaffolds of triblock PEG-PCL-PEG copolymer fabricated by an air pressure-aided deposition system. A desktop air pressure-aided deposition system that involves melting and plotting PEG-PCL-PEG was used to fabricate three-dimensional scaffolds having rectangular pores. The experimental results showed that PEG-PCL-PEG with a molecular weight of 25,000 can be melted and stably deposited through a heating nozzle at an air pressure of 0.3?MPa and no crack occurs after it solidifies. The scaffolds with pre-determined pore size of 400× 420?μm and a porosity of 79?% were fabricated, and their average compressive strength was found to be 18.2?MPa. Osteoblast-like cells, MC3T3-E1, were seeded on fabricated scaffolds to investigate the in-vitro response of cells including toxicity and cellular locomotion. In a culture period of 28?days, the neutral-red stained osteoblasts were found to well distributed in the interior of the scaffold. Furthermore, the cellular attachment and movement in the first 10?h of cell culture were observed with time-lapse microscopy indicating that the porous PEG-PCL-PEG scaffolds fabricated by air pressure-aided deposition system is non-toxicity for osteoblast-like cells.