Establishment of an Agrobacterium-mediated genetic transformation procedure for the experimental model orchid Erycina pusilla

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• 作者：Shu-Hong Lee (1) (2)
  Chia-Wen Li (3)
  Chia-Hui Liau (2)
  Pao-Yi Chang (2)
  Li-Jen Liao (4)
  Choun-Sea Lin (5)
  Ming-Tsair Chan (1) (2) (5)
  
• 关键词：Methionine sulfoxide reductase ; Methyl viologen ; Orchid
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：120
• 期：1
• 页码：211-220
• 全文大小：1,570 KB
• 参考文献：1. Chan MT, Chan YL, Sanjaya (2007) Orchids ( / Cymbidium spp., / Oncidium, and / Phalaenopsis). In: Wang K (ed) / Agrobacterium protocols volume 2, vol 344. Methods in Molecular Biology. Humana Press, pp 331-38
  2. Chang YY, Chiu YF, Wu JW, Yang CH (2009) Four orchid ( / Oncidium Gower Ramsey) / AP1/AGL9- / like MADS box genes show novel expression patterns and cause different effects on floral transition and formation in / Arabidopsis thaliana. Plant Cell Physiol 50(8):1425-438 CrossRef
  3. Chang YY, Kao NH, Li JY, Hsu WH, Liang YL, Wu JW, Yang CH (2010) Characterization of the possible roles for B class / MADS box genes in regulation of perianth formation in orchid. Plant Physiol 152(2):837-53 CrossRef
  4. Girhepuje PV, Shinde GB (2011) Transgenic tomato plants expressing a wheat endochitinase gene demonstrate enhanced resistance to / Fusarium oxysporum f. sp. / lycopersici. Plant Cell Tiss Organ Cult 105:243-51 CrossRef
  5. Guo B, Hexige S, Zhang T, Pittman JK, Chen D, Ming F (2007) Cloning and characterization of a / PI- / like MADS- / box gene in / Phalaenopsis orchid. J Biochem Mol Biol 40(6):845-52 CrossRef
  6. Hsu HF, Huang CH, Chou LT, Yang CH (2003) Ectopic expression of an orchid ( / Oncidium Gower Ramsey) / AGL6- / like gene promotes flowering by activating flowering time genes in / Arabidopsis thaliana. Plant Cell Physiol 44(8):783-94 CrossRef
  7. Hsu CT, Liao DC, Wu FH, Liu NT, Shen SC, Chou SJ, Tung SY, Yang CH, Chan MT, Lin CS (2011) Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of / Oncidium Gower Ramsey. BMC Plant Biol 11:60 CrossRef
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  10. Kwon SJ, Kwon SI, Bae MS, Cho EJ, Park OK (2007) Role of the methionine sulfoxide reductase MsrB3 in cold acclimation in / Arabidopsis. Plant Cell Physiol 48(12):1713-723 CrossRef
  11. Lee SH, Li CW, Koh KW, Chuang HY, Chen YR, Lin CS, Chan MT (2014) MSRB7 reverses oxidation of GSTF2/3 to confer tolerance of A / rabidopsis thaliana to oxidative stress. J Exp Bot. doi:10.1093/jxb/eru270
  12. Li CW, Su RC, Cheng CP, Sanjaya, You SJ, Hsieh TH, Chao TC, Chan MT (2011) Tomato RAV transcription factor is a pivotal modulator involved in the AP2/EREBP-mediated defense pathway. Plant Physiol 156(1):213-27 CrossRef
  13. Li CW, Lee SH, Chieh PS, Lin CS, Wang YC, Chan MT (2012) / Arabidopsis root-abundant cytosolic / methionine sulfoxide reductase B genes MsrB7 and / MsrB8 are involved in tolerance to oxidative stress. Plant Cell Physiol 53(10):1707-719 CrossRef
  14. Li CW, Lee SH, Chan MT (2013) Utilization of the plant methionine sulfoxide reductase B genes as selectable markers in / Arabidopsis and tomato transformation. Plant Cell Tiss Organ Cult 113(3):555-63 CrossRef
  15. Liao LJ, Pan IC, Chan YL, Hsu YH, Chen WH, Chan MT (2004) Transgene silencing in / Phalaenopsis expressing the coat protein of / Cymbidium Mosaic Virus is a manifestation of RNA-mediated resistance. Mol Breed 13(3):229-42 CrossRef
  16. Liau CH, Lu JC, Prasad V, Hsiao HH, You SJ, Lee JT, Yang NS, Huang HE, Fe
• 作者单位：Shu-Hong Lee (1) (2)
  Chia-Wen Li (3)
  Chia-Hui Liau (2)
  Pao-Yi Chang (2)
  Li-Jen Liao (4)
  Choun-Sea Lin (5)
  Ming-Tsair Chan (1) (2) (5)
  
  1. Institute of Biotechnology, National Cheng Kung University, Tainan, 701, Taiwan
  2. Academia Sinica Biotechnology Center in Southern Taiwan, Tainan, 741, Taiwan
  3. Department of Biotechnology, TransWorld University, Douliu City, Yunlin County, 640, Taiwan
  4. Institute of Life Science, National Kaohsiung Normal University, Kaohsiung, 802, Taiwan
  5. Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 115, Taiwan
  
• ISSN：1573-5044

文摘

The establishment of a genetic transformation system for Erycina pusilla (E. pusilla) would be a major step for orchid research and help establish the species as a potential experimental model. Here we describe the development of an efficient selection and plant regeneration protocol for Agrobacterium-mediated transformation of E. pusilla protocorms. Plants were transformed with a binary plasmid containing the Arabidopsis thaliana gene Methionine sulfoxide reductase B7 (MSRB7) using the A. tumefaciens strain EHA105. Explants were selected on hygromycin-containing New Dogashima medium (Tokuhara and Mii in Plant Cell Rep 13(1):7-1, 1993) with 6-benzylaminopurine and α-naphthaleneacetic acid for 5?months to diminish the ratio of false positive explants, and it was determined that 3?month-old E. pusilla protocorms represented the optimal stage for transformation. Positive transformants were confirmed by Southern blot and PCR analyses, and by methyl viologen tolerance assays. Since E. pusilla has a shorter life cycle than most other orchids, self-pollinated T1 progeny was obtained within only 18?months. In addition, the results indicated that overexpression of MSRB genes can be used to avoid the effects of abiotic stress in economically important plants such as orchids.