In situ molecular self-assembly and sensitive label-free detection of streptavidin via a wavelength interrogated surface plasmon resonance sensor

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• 作者：Yong-jiang Yu (1)
  Ying Zhou (1)
  Qiu-shun Li (2)
  Yan Yang (2)
  Jian-guo Shi (2)
  Ming-yu Li (3)
  Wei-guo Yao (4)
  Jun-nan Wang (5)
  Wen-fei Dong (1)
  Zhi-mei Qi (6)
  
• 关键词：Polyelectrolyte multilayer ; Sensing sensitivity ; Biosensor ; Wavelength interrogated surface plasmon resonance
• 刊名：Chemical Research in Chinese Universities
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：29
• 期：6
• 页码：1219-1224
• 全文大小：939 KB
• 作者单位：Yong-jiang Yu (1)
  Ying Zhou (1)
  Qiu-shun Li (2)
  Yan Yang (2)
  Jian-guo Shi (2)
  Ming-yu Li (3)
  Wei-guo Yao (4)
  Jun-nan Wang (5)
  Wen-fei Dong (1)
  Zhi-mei Qi (6)
  
  1. State Key Laboratory on Integrated Optoelectronics, College of Integrated Optoelectronics, Jilin University, Changchun, 130012, P. R. China
  2. Key Biosensor Laboratory of Shandong Province, Biology Institute of Shandong Academy of Sciences, Jinan, 250014, P. R. China
  3. State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou, 310027, P. R. China
  4. Key Laboratory of Automobile Materials, Ministry of Education, Department of Materials Science and Engineering, Jilin University, Changchun, 130025, P. R. China
  5. The Second Hospital of Jilin University, Changchun, 130041, P. R. China
  6. State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, 100190, P. R. China
  
• ISSN：2210-3171

文摘

The sensing sensitivity of wavelength interrogated surface plasmon resonance(WISPR) biosensor is improved by self-assembly of polyelectrolyte multilayer(PEM) film of poly(allylamine hydrochloride)(PAH)/poly(sodium-p-styrenesulfonate)(PSS) on the Au film coated glass chip via the layer-by-layer(LBL) technique. The home-made WISPR with Krestchmann configuration consists of a tungsten-halogen lamp as a photon source and a charge coupled device(CCD) camera as the detector. The influence of PEM film thickness on the optical properties of WISPR biosensors was investigated theoretically and experimentally. In order to achieve higher sensing sensitivity, the PEM film thickness has to be designed as ca.14 nm at an Au layer thickness of 50 nm and an incidental angle of 11.8°. Furthermore, the PEM coated WISPR biosensor can serve as highly sensitive biosensor, in which the biotin-streptavidin is used as bioconjugate pair. After deposition of the PEM film of (biotin/PAH)(PSS/PAH)3, the modified WISPR biosensor is more sensitive to the low concentration(<0.01 mg/mL) of streptavidin. And the sensing sensitivity can be further increased by one order of magnitude compared with that of the biotin/PAH coated WISPR biosensor. Thus, such low-cost, high-performance and efficient PEM-coated WISPR biosensors have great potentials in a diverse array of fields such as medical diagnostics, drug screening, food safety analysis, environmental monitoring, and homeland security.