Ratio of γ-H2AX level in lymphocytes to that in granulocytes detected using flow cytometry as a potential biodosimeter for radiation exposure

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• 作者：Zhidong Wang (1)
  Hailiang Hu (1)
  Ming Hu (1)
  Xueqing Zhang (1)
  Qi Wang (2)
  Yulei Qiao (1)
  Haixiang Liu (1)
  Liping Shen (1)
  Pingkun Zhou (1)
  Ying Chen (1)
  
• 关键词：RL/G of γ ; H2AX ; Radiation ; Lymphocyte ; Granulocyte ; Flow cytometry
• 刊名：Radiation and Environmental Biophysics
• 出版年：2014
• 出版时间：May 2014
• 年：2014
• 卷：53
• 期：2
• 页码：283-290
• 全文大小：
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• 作者单位：Zhidong Wang (1)
  Hailiang Hu (1)
  Ming Hu (1)
  Xueqing Zhang (1)
  Qi Wang (2)
  Yulei Qiao (1)
  Haixiang Liu (1)
  Liping Shen (1)
  Pingkun Zhou (1)
  Ying Chen (1)
  
  1. Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, China
  2. Beijing Consulting Centre of Biomedical Statistics, 27 Taiping Road, Beijing, 100850, China
  
• ISSN：1432-2099

文摘

This study aims to assess utilisation of the ratio of γ-H2AX in lymphocytes to that in granulocytes (RL/G of γ-H2AX) in blood as a rapid method for population triage and dose estimation during large-scale radiation emergencies. Blood samples from healthy volunteers exposed to 0-0?Gy of 60Co irradiation were collected. The samples were cultured for 0-4?h and then analysed using flow cytometry to measure the levels of γ-H2AX in lymphocytes and granulocytes. The basal RL/G levels of γ-H2AX in healthy human blood, the response of RL/G of γ-H2AX to ionising radiation and its relationship with doses, time intervals after exposure and individual differences were also analysed. The level of γ-H2AX in lymphocytes increased in a dose-dependent manner after irradiation, whereas the level in granulocytes was not affected. A linear dose–effect relationship with low inter-experimental and inter-individual variations was observed. The RL/G of γ-H2AX may be used as a biomarker for population triage and dose estimation during large-scale radiation emergencies if blood samples can be collected within 24?h.