Synthesis of peptide templated copper nanoclusters for fluorometric determination of Fe(III) in human serum

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• 作者：Ting Tang ; Jiang Ouyang ; Lanshuang Hu ; Linyan Guo ; Minghui Yang…
• 关键词：Nanomaterial ; Template synthesis ; Fluorescence ; Quenching ; Serum analysis
• 刊名：Microchimica Acta
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：183
• 期：10
• 页码：2831-2836
• 全文大小：642 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Inorganic Chemistry
  Physical Chemistry
  Characterization and Evaluation Materials
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Wien
• ISSN：1436-5073
• 卷排序：183

文摘

Copper nanoclusters (Cu-NCs) were prepared by reducing CuCl₂ with ascorbic acid in the presence of the short peptide template Cys-Cys-Cys-Asp-Leu. They were characterized by UV-vis absorption and fluorescence spectroscopy, transmission electron microscopy and X-ray photoelectron spectroscopy. The Cu-NCs have a size of ~2 nm, can be well dispersed in water and are photostable. Their fluorescence (peaking at 425 nm under 365-nm excitation) is quenched by Fe(III) ions. Based on this finding, a sensitive and selective fluorescence assay for the detection of Fe(III) was developed. Under optimized conditions and a pH value of 2.0, the assay displays a linear response in the 0.05 to 30 μM Fe(III) concentration range, with a detection limit of 20 nM based on an S/N ratio of 3. The assay was successfully applied to the determination of Fe(III) in spiked human serum where is gave recoveries that ranged from 96.2 % to 98.3 %.