Identification of oleaginous yeast strains able to accumulate high intracellular lipids when cultivated in alkaline pretreated corn stover
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- 作者：Irnayuli R. Sitepu (1) (2)
Mingjie Jin (3)
J. Enrique Fernandez (1)
Leonardo da Costa Sousa (3)
Venkatesh Balan (3)
Kyria L. Boundy-Mills (1) - 关键词：Lignocellulosic ; Cryptococcus humicola ; Biodiesel ; Energy ; Oleochemics
- 刊名：Applied Microbiology and Biotechnology
- 出版年：2014
- 出版时间：September 2014
- 年：2014
- 卷：98
- 期：17
- 页码：7645-7657
- 全文大小：576 KB
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Mingjie Jin (3)
J. Enrique Fernandez (1)
Leonardo da Costa Sousa (3)
Venkatesh Balan (3)
Kyria L. Boundy-Mills (1)
1. Phaff Yeast Culture Collection, Department of Food Science and Technology, University of California, One Shields Avenue, Davis, CA, 95616, USA
2. Forestry Research and Development Agency (FORDA), The Ministry of Forestry, Jalan Gunung Batu No. 5, Bogor, 16610, Indonesia
3. Biomass Conversion Research Laboratory, Department of Chemical Engineering and Material Science, Michigan State University, East Lansing, MI, USA - ISSN：1432-0614
文摘
Microbial oil is a potential alternative to food/plant-derived biodiesel fuel. Our previous screening studies identified a wide range of oleaginous yeast species, using a defined laboratory medium known to stimulate lipid accumulation. In this study, the ability of these yeasts to grow and accumulate lipids was further investigated in synthetic hydrolysate (SynH) and authentic ammonia fiber expansion (AFEX?-pretreated corn stover hydrolysate (ACSH). Most yeast strains tested were able to accumulate lipids in SynH, but only a few were able to grow and accumulate lipids in ACSH medium. Cryptococcus humicola UCDFST 10-1004 was able to accumulate as high as 15.5?g/L lipids, out of a total of 36?g/L cellular biomass when grown in ACSH, with a cellular lipid content of 40?% of cell dry weight. This lipid production is among the highest reported values for oleaginous yeasts grown in authentic hydrolysate. Preculturing in SynH media with xylose as sole carbon source enabled yeasts to assimilate both glucose and xylose more efficiently in the subsequent hydrolysate medium. This study demonstrates that ACSH is a suitable medium for certain oleaginous yeasts to convert lignocellullosic sugars to triacylglycerols for production of biodiesel and other valuable oleochemicals.