Isolation, molecular characterization and evaluation of the pathogenicity of a porcine rotavirus isolated from Jiangsu Province, China

详细信息    查看全文

• 作者：Hewei Zhang ; Zhuo Zhang ; Yufei Wang ; Xin Wang ; Mingqi Xia ; Hua Wu
• 刊名：Archives of Virology
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：160
• 期：5
• 页码：1333-1338
• 全文大小：971 KB
• 参考文献：1. Zhen, Yin, Jing-hua, Liu (1997) Animal Virology. Science Press, Beijing
  2. Saikruang, W, Khamrin, P, Chaimongkol, N (2013) Genetic diversity and novel combinations of G4P[19] and G9P[19] porcine rotavirus strains in Thailand. Vet Microbiol 161: pp. 255-262 CrossRef
  3. Frederick, AM (1999) Veterinary virology. Academic Press, California
  4. Tam, JS, Szymanski, MT, Middleton, PJ, Petric, M (1989) Epidemiological studies of piglet diarrhea in intensively managed Danish sow herds. IV. Pathogenicity of porcine rotavirus. Acta Vet Scand 30: pp. 71-76
  5. Estes, MK, Cohen, J (1989) Rotavirus gene structure and function. Microbiol Rev 53: pp. 410-449
  6. Estes, MK, Kang, G, Zeng, CQ, Crawford, SE, Ciarlet, M (2001) Pathogenesis of rotavirus gastroenteritis. Novartis Found Symp 238: pp. 82-96 CrossRef
  7. Gorziglia, M, Larralde, G, Kapikian, AZ, Chanock, RM (1990) Antigenic relationgships among human rotaviruses as determined by outer capsid protein VP4. Proc Natl Acad Sci USA 87: pp. 7155-7159 CrossRef
  8. Jiang, BM, Saif, LJ, Kang, SY, Kim, JH (1990) Biochemical characterization of the sturctural and nonstructural polypeptides of a porcine group C rotavirus. J Virol 64: pp. 3171-3178
  9. Ahmadian, S, Shahrabadi, MS (1999) Morphological study of the role of calcium in the assembly of the rotavirus outer capsid protein VP7. Biotech Histochem 74: pp. 266-273 CrossRef
  10. Pedley, S, Bridger, JC, Chasey, D, McCrae, MA (1986) Definition of two new groups of atypical rotaviruses. J Gen Virol 67: pp. 131-137 CrossRef
  11. Fu, ZF, Hampson, DJ, Blackmore, DK (1989) Detection and survival of group A rotavirus in a piggery. Vet Rec 125: pp. 576-578
  12. Saif LJ, Azevedo MSP, Yuan L, Jeong KI, Nguyen TV, Pouly SM, Gochnauer M (2003) Nasal and rectal shedding and viremia in gnotobiotic piglets after oral or intranasal inoculation with Wa human rotavirus. 8th International Symposium on ds-RNA Viruses. Abstract W6.3
  13. Woode, GN (1978) Epizootiology of bovine rotavirus infection. Vet Rec 103: pp. 44-46 CrossRef
  14. Bohl, EH, Theil, KW, Saif, LJ (1984) Isolation and serotyping of porcine rotaviruses and antigenic comparison with other rotaviruses. J Clin Microbiol 19: pp. 105-111
  15. Lin, HT, Tsai, HY, Liu, CP (2010) Comparability of bovine virus titers obtained by TCID50/ml and FAID50/ml. J Virol Methods 165: pp. 121-124 CrossRef
  16. Larralde, G, Gorziglia, M (1992) Distribution of conserved and specific epitopes on the VP8 subunit of rotavirus VP4. J Virol 66: pp. 7438-7443
  17. Stacy, AS, Gavan, H, Barbara, SC (2005) Crystallization and preliminary X-ray diffraction analysis of the sialic acid-binding domain (VP8*) of porcine rotavirus strain CR W-8. Acta crystallogr Sect F Struct Biol Cryst Commun 61: pp. 617-620 CrossRef
  18. Ghosh, S, Kobayashi, N, Nagashima, S (2010) Full genomic analysis and possible origin of a porcine G12 rotavirus strain RU172. Virus Genes 40: pp. 382-388 CrossRef
  19. Ciarlet, M, Liprandi, F (1994) Serological and genomic characterization of two porcine rotavirus with serotype G specificity. J clin Microbiol 32: pp. 269-272
  20. Alfieri, AA, Leite, JP (1996) Characterization of human rotaivirus genotype P[8]G[5] from Brazil by probehy bridization and sequence. Arch Virol 141: pp. 2353-2364 CrossRef
  21. Hajnalka, P, Brigitta, L, Ferenc, J (2013) Review of group A rotavirus strains reported in swine and cattle. Vet Microbiol 165: pp. 190-199 CrossRef
  22. Sattar, SA, Jacobsen, H, Rahman, H (1994) Interruption of rotavirus spread through chemical disinfection. Infect Control Hosp Epidemiol 15: pp. 751
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Virology
  Medical Microbiology
  Infectious Diseases
  
• 出版者：Springer Wien
• ISSN：1432-8798

文摘

In this study, a porcine rotavirus was isolated from a fecal sample from a diarrheic piglet in Jiangsu Province, China. Rotavirus-specific cytopathic effects were observed after 12 blind passages on MA-104 cells, yielding a virus titer of 106.125 TCID50/ml. By applying an 80?% nucleotide cutoff value and the RotaC2.0 automated genotyping tool, the Vp4 genotype of the new isolate was identified as P[7]. The Vp7 genotype was identified as G[9], lineage VI, and sublineage c. Experimentally infected piglets showed severe diarrhea symptoms 16-4?h post-inoculation, indicating that this new porcine rotavirus isolate is a pathogenic strain.