An Electrochemical Method for High Sensitive Detection of Thiabendazole and Its Interaction with Human Serum Albumin

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• 作者：Jichun Yang ; Danfeng Zhang ; Lingling Wang ; Na Long ; Minhui Zhang…
• 关键词：Thiabendazole ; Human serum albumin ; Carboxyl ; functionalized multi ; walled carbon nanotubes ; Interaction ; Differential pulse voltammetry
• 刊名：Food Analytical Methods
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：8
• 期：2
• 页码：507-514
• 全文大小：1,621 KB
• 参考文献：1. Bard AJ, Faulkner LR (2001) Electrochemical methods: fundamentals and applications, 2nd edn. Wiley, New York
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Chemistry
  Microbiology
  Analytical Chemistry
  
• 出版者：Springer New York
• ISSN：1936-976X

文摘

In this study, a simple and highly sensitive electroanalytical method for the determination of thiabendazole (TBZ) was developed using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) at carboxyl-functionalized multi-walled carbon nanotubes (MWCNT-COOH) modified glassy carbon electrode (MWCNT-COOH/GCE) in phosphate buffer solution (pH--.0). MWCNT-COOH/GCE exhibits an enhanced effectiveness for the redox of TBZ. This constructed biosensor exhibited two linear relationships with TBZ concentration range from 5.00?×-0? to 4.95?×-0??mol/L and 4.95?×-0? to 9.80?×-0??mol/L, respectively. The detection limit was 3.0?×-0??mol/L (S/N--) and the relative standard deviation (R.S.D.) was lower than 4.2?% (n--). The proposed method was successfully applied to determine TBZ in real samples and the results were satisfactory. The MWCNT-COOH/GCE electrode showed good reproducibility, stability, and anti-interference. The electrochemistry method was also described for the evaluation of TBZ and human serum albumin (HSA) interaction. In the presence of HSA, the peak currents of TBZ decreased linearly due to the formation of a kind of electro-inactive complex. The binding constant between TBZ and HSA was obtained by DPV.