Enhancement of lipid productivity by ethyl methane sulfonate-mediated random mutagenesis and proteomic analysis in Chlamydomonas reinhardtii
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- 作者：Bongsoo Lee (1)
Gang-Guk Choi (1)
Yoon-E. Choi (2)
Minji Sung (1)
Min S. Park (1) (3)
Ji-Won Yang (1) (3) - 关键词：Ethyl Methane Sulfonate (EMS) ; Random Mutagenesis ; Chlamydmonas reinhardtii ; Lipid Productivity ; Proteomics
- 刊名：Korean Journal of Chemical Engineering
- 出版年：2014
- 出版时间：June 2014
- 年：2014
- 卷：31
- 期：6
- 页码：1036-1042
- 全文大小：
- 参考文献：1. P. McKendry, / Bioresour. Technol., 83, 37 (2002). CrossRef
2. P. S. Nigam and A. Singh, / Prog. Energy Combust. Sci., 37, 52 (2011). CrossRef
3. T. M. Mata, A. A. Martins and N. S. Caetano, / Renewable Sustainable Energy Rev., 14, 217 (2010). CrossRef
4. Y. Chisti, / Biotechnol. Adv., 25, 294 (2007). CrossRef
5. T. Minowa, S.-y. Yokoyama, M. Kishimoto and T. Okakura, / Fuel, 74, 1735 (1995). CrossRef
6. A. Ahmad, N. Yasin, C. Derek and J. Lim, / Renewable Sustainable Energy Rev., 15, 584 (2011). CrossRef
7. P. T. Pienkos and A. Darzins, / Biofuels, Bioprod. Biorefin., 3, 431 (2009). CrossRef
8. H. M. Amaro, A. Guedes and F. X. Malcata, / Appl. Energy, 88, 3402 (2011). CrossRef
9. H. Rismani-Yazdi, B. Z. Haznedaroglu, C. Hsin and J. Peccia, / Biotechnol. Biofuels, 5, 1 (2012). CrossRef
10. R. Radakovits, R. E. Jinkerson, S. I. Fuerstenberg, H. Tae, R. E. Settlage, J.L. Boore and M. C. Posewitz, / Nat. Commun., 3, 686 (2012). CrossRef
11. S. S. Merchant, S. E. Prochnik, O. Vallon, E. H. Harris, S. J. Karpowicz, G.B. Witman, A. Terry, A. Salamov, L. K. Fritz-Laylin and L. Maréchal-Drouard, / Science, 318, 245 (2007). CrossRef
12. A. Z. Worden, J.-H. Lee, T. Mock, P. Rouzé, M. P. Simmons, A. L. Aerts, A. E. Allen, M. L. Cuvelier, E. Derelle and M.V. Everett, / Science, 324, 268 (2009). CrossRef
13. A. Eichler-Stahlberg, W. Weisheit, O. Ruecker and M. Heitzer, / Planta, 229, 873 (2009). CrossRef
14. R. Radakovits, R. E. Jinkerson, A. Darzins and M. C. Posewitz, / Eukaryotic Cell, 9, 486 (2010). CrossRef
15. U.W. Goodenough, / Cell, 70, 533 (1992). CrossRef
16. E. H. Harris, / Annu. Rev. Plant Biol., 52, 363 (2001). CrossRef
17. B. Zorin, Y. Lu, I. Sizova and P. Hegemann, / Gene, 432, 91 (2009). CrossRef
18. L. L. Beer, E. S. Boyd, J.W. Peters and M. C. Posewitz, / Curr. Opin. Biotechnol., 20, 264 (2009). CrossRef
19. M. Mobini-Dehkordi, I. Nahvi, H. Zarkesh-Esfahani, K. Ghaedi, M. Tavassoli and R. Akada, / J. Biosci. Bioeng., 105, 403 (2008). CrossRef
20. Y. Li, D. Han, G. Hu, M. Sommerfeld and Q. Hu, / Biotechnol. Bioeng., 107, 258 (2010). CrossRef
21. V. H. Work, R. Radakovits, R. E. Jinkerson, J. E. Meuser, L. G. Elliott, D. J. Vinyard, L. M. Laurens, G. C. Dismukes and M. C. Posewitz, / Eukaryotic Cell, 9, 1251 (2010). CrossRef
22. M. H. Huesemann, T. S. Hausmann, R. Bartha, M. Aksoy, J. C. Weissman and J. R. Benemann, / Appl. Biochem. Biotechnol., 157, 507 (2009). CrossRef
23. Y.-H. Kim, H.-J. Park, S.-H. Lee and J.-H. Lee, / Korean J. Chem. Eng., 30, 413 (2013). CrossRef
24. K. Anandarajah, G. Mahendraperumal, M. Sommerfeld and Q. Hu, / Appl. Energy, 96, 371 (2012). CrossRef
25. W. Chen, C. Zhang, L. Song, M. Sommerfeld and Q. Hu, / J. Microbiol. Methods, 77, 41 (2009). CrossRef
26. M.M. Bradford, / Anal. Biochem., 72, 248 (1976). CrossRef
27. A. Shevchenko and A. Shevchenko, / Anal. Biochem., 296, 279 (2001). CrossRef
28. Y. Li, M. Horsman, B. Wang, N. Wu and C.Q. Lan, / Appl. Microbiol. Biotechnol., 81, 629 (2008). CrossRef
29. Z. T. Wang, N. Ullrich, S. Joo, S. Waffenschmidt and U. Goodenough, / Eukaryotic Cell, 8, 1856 (2009). CrossRef
30. T. Govender, L. Ramanna, I. Rawat and F. Bux, / Bioresour. Technol., 114, 507 (2012). CrossRef
31. M. S. Cooper, W. R. Hardin, T.W. Petersen and R. A. Cattolico, / J. Biosci. Bioeng., 109, 198 (2010). CrossRef
32. R. Sager and S. Granick, / J. Gen. Physiol., 37, 729 (1954). CrossRef
33. R. Singh, S. Kaushik, Y. Wang, Y. Xiang, I. Novak, M. Komatsu, K. Tanaka, A. M. Cuervo and M. J. Czaja, / Nature, 458, 1131 (2009). CrossRef
34. K. K. Sharma, H. Schuhmann and P.M. Schenk, / Energies, 5, 1532 (2012). CrossRef
35. Q. Hu, M. Sommerfeld, E. Jarvis, M. Ghirardi, M. Posewitz, M. Seibert and A. Darzins, / Plant J., 54, 621 (2008). CrossRef
36. P. I. Leonardi, C. A. Popovich and M. C. Damiani, / Econ. Eff. Biofuels. Prod. (2011).
37. A. Converti, A. A. Casazza, E.Y. Ortiz, P. Perego and M. Del Borghi, / Chem. Eng. Process., 48, 1146 (2009). CrossRef
38. G. O. James, C. H. Hocart, W. Hillier, H. Chen, F. Kordbacheh, G. D. Price and M. A. Djordjevic, / Bioresour. Technol., 102, 3343 (2011). CrossRef
39. W. Majeran, J. Olive, D. Drapier, O. Vallon and F.-A. Wollman, / Plant Physiol., 126, 421 (2001). CrossRef
40. K. S. Ishimoto and S. Lory, / J. Bacteriol., 174, 3514 (1992).
41. I.M. Ota and S. Lory, / Science, 262, 566 (1993). CrossRef
42. C. Chang, S. F. Kwok, A. B. Bleecker and E.M. Meyerowitz, / Science, 262, 539 (1993). CrossRef
43. G. E. Schaller, S.-H. Shiu and J. P. Armitage, / Curr. Biol., 21, R320 (2011). CrossRef - 作者单位：Bongsoo Lee (1)
Gang-Guk Choi (1)
Yoon-E. Choi (2)
Minji Sung (1)
Min S. Park (1) (3)
Ji-Won Yang (1) (3)
1. Department of Chemical and Biomolecular Engineering, KAIST, Yuseong-gu, Daejeon, 305-701, Korea
2. LED Agri-bio Fusion Technology Research Center, Chonbuk National University, 79, Gobong-ro, Iksan-si, Jeollabuk-do, 570-752, Korea
3. Advanced Biomass R&D Center, KAIST, Yuseong-gu, Daejeon, 305-701, Korea - ISSN：1975-7220
文摘
Microalgae-derived biomass has been considered as the most promising candidate for next generation biofuel due to its sustainability and biodegradability. In this study, microalgal strain Chlamydmonas reinhardtii was randomly mutagenized by using a chemical mutagen, ethyl methane sulfonate (EMS) to create mutants showing enhanced lipid production. We identified three random mutants that displayed high lipid production in the screening using Nile red staining. Among those, mutant #128 was selected as candidate for further studies. Our flow cytometry and confocal microscopy analysis revealed that mutant #128 contains larger and more abundant lipid bodies than that of wild-type. Moreover, mutant #128 showed 1.4-fold increased fatty acid methyl ester (FAME) content compared to wild-type under nitrogen depleted condition. In addition, mutant #128 grew faster and accumulated more biomass, resulting in high lipid production. 2D gel electrophoresis and MALDI-TOF analysis used for gene targeting revealed that β-subunit of mitochondrial ATP Synthase and two-component response regulator PilR may be involved in enhanced characteristics of mutant #128. These results show the possibilities of EMS mediated random mutagenesis in generation of mutants to produce high amount of lipid as well as further study for molecular mechanism of mutants.