Anti-inflammatory effects of Polygonum minus (Huds) extract (Lineminus? in in-vitro enzyme assays and carrageenan induced paw edema

详细信息    查看全文

• 作者：Annie George ; Sasikala Chinnappan…
• 关键词：Inflammation ; Polygonum minus (Huds) ; Paw edema ; Cyclooxygenase ; Lipooxygenase ; Secretory phospholipase ; A2
• 刊名：BMC Complementary and Alternative Medicine
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：14
• 期：1
• 全文大小：303 KB
• 参考文献：1. Michaelsson, E, Holmdahl, M, Engstrom, A, Burkhardt, H, Scheynius, A, Holmdahl, R (1995) Macrophages, but not dendritic cells, present collagen to T cells. Eur J Immunol 25: pp. 234-2241
  2. Stuhlmuller, B, Ungethum, U, Scholze, S, Martinez, L, Backhaus, M, Kraetsch, HG, Kinne, RW, Burmester, GR (2000) Identification of known and novel genes in activated monocytes from patients with rheumatoid arthritis. Arthritis Rheum 43: pp. 775-790 href="http://dx.doi.org/10.1002/1529-0131(200004)43:4<775::AID-ANR8>3.0.CO;2-7" target="_blank" title="It opens in new window">CrossRef
  3. Ricciotti, E, FitzGerald, GA (2011) Prostaglandins and Inflammation. Arterioscler Thromb Vasc Biol 31: pp. 986-1000 href="http://dx.doi.org/10.1161/ATVBAHA.110.207449" target="_blank" title="It opens in new window">CrossRef
  4. Namgaldaze, D, Bruene, B (2006) Phospholipase A2–modified LDL activates the phosphatidylinositol3-kinase-akt pathway and increases cell survival in monocyte cells. Arterioscler Thromb Vasc Biol 26: pp. 2510-2516 href="http://dx.doi.org/10.1161/01.ATV.0000245797.76062.2e" target="_blank" title="It opens in new window">CrossRef
  5. Oestvang, J, Johansen, B (2006) Phospholipase A2: A keyregulator of inflammatory signaling and a corrector to fibrosis development in atherosclerosis. BBA- Mol Cell Biol L 176: pp. 1309-1316
  6. Xie, WL, Robertson, DL, Simmons, DL (1992) Mitogen-inducible prostaglandin G/H synthase -a new target for nonsteroidal anti inflammatory drugs. Drug Dev Res 25: pp. 249-265 href="http://dx.doi.org/10.1002/ddr.430250402" target="_blank" title="It opens in new window">CrossRef
  7. Jia, Q, Nichols, TC, Rhoden, EE, Waite, S (2003) Identification of Free-B-Ring Flavonoids as Potent COX-2 Inhibitors.
  8. Vecka, M, Prokes, L, Tvrzicka, E, Karpas, K, Pernicky, A, Pfleger, R, Votruba, M (2008) Anti-inflammatory effect of flavonoids from Comfort-G and the changes in arachidonic acid metabolism. Klin Biochem Metab 16: pp. 27-32
  9. Zarghi, A, Arfaei, S (2011) Selective COX-2 inhibitors: a review of their structure-activity relationships. Iran J Pharm Res 10: pp. 655-683
  10. Aggarwal, BB, Shishodia, S, Sandur, SK, Pandey, MK, Sethi, G (2006) Inflammation and cancer: How hot is the link?. Biochem Pharmacol 72: pp. 1605-1621 href="http://dx.doi.org/10.1016/j.bcp.2006.06.029" target="_blank" title="It opens in new window">CrossRef
  11. Miyahara, N, Miyahara, S, Takeda, K, Gelfandet, EW (2006) Role of the LTB4/BLT1 pathway in allergen-induced airway hyperresponsiveness and inflammation. Allergo Int 55: pp. 91-97 href="http://dx.doi.org/10.2332/allergolint.55.91" target="_blank" title="It opens in new window">CrossRef
  12. Stamatovic, SM, Dimitrijevic, OB, Keep, RF, Andjelkovic, AV (2006) Inflammation and brain edema: new insights into the role of chemokines and their receptors. Acta Neurochir Suppl 96: pp. 444-450 href="http://dx.doi.org/10.1007/3-211-30714-1_91" target="_blank" title="It opens in new window">CrossRef
  13. Chrubasik, JE, Roufogalis, BD, Chrubasik, S (2007) Evidence of effectiveness of herbal anti inflammatory drugs in the treatment of painful osteoarthritis and chronic low back pain. Phytother Res 21: pp. 675-683 href="http://dx.doi.org/10.1002/ptr.2142" target="_blank" title="It opens in new window">CrossRef
  14. Naesdal, J, Brown, K (2006) NSAID associated adverse effects and acid control aids to prevent them: A review of current treatment options. Drug Saf 29: pp. 119-132 href="http://dx.doi.org/10.2165/00002018-200629020-00002" target="_blank" title="It opens in new window">CrossRef
  15. Salmon, SD (2006) Cyclooxygenase-2 inhibitors and cardiovascular risk. Curr Opin Cardiol 21: pp. 613-617
  16. Greenberg, JD, Fisher, MC, Kremer, J, Chang, H, Rosentein, ED, Kishimoto, M, Lee, S, Yazici, Y, Kavanaugh, A, Abramson, SB (2009) The COX-2 inhibitors market withdrawals and prescribing patterns by rheumatologist in patients with gastrointestinal and cardiovascular risk. Clin Exp Rheumatol 27: pp. 395-401
  17. Sengupta, K, Alluri, KV, Satish, AR, Mishras, S, Golakoti, T, Sarma, KV, Dey, D, Raychaudhuri, SP (2008) A double blind, randomized, placebo controlled study of the efficacy and safety of 5-Loxin? for treatment of osteoarthritis of the knee. Arthritis Res Ther 10: pp. 85 href="http://dx.doi.org/10.1186/ar2461" target="_blank" title="It opens in new window">CrossRef
  18. Oliver, W (2007) Inhibition of 5-Lipoxygenase product synthesis by natural compounds of plant origin. Planta Med 73: pp. 1331-1357 href="http://dx.doi.org/10.1055/s-2007-990242" target="_blank" title="It opens in new window">CrossRef
  19. Burkill, HI (1966) A Dictionary of the Economic Prod
• 刊物主题：Complementary & Alternative Medicine; Internal Medicine; Chiropractic Medicine;
• 出版者：BioMed Central
• ISSN：1472-6882

文摘

Background The study was aimed to evaluate the anti-inflammatory activity of ethanolic and aqueous extracts of Polygonum minus (Huds) using in vitro and in vivo approaches. Methods The in vitro tests used to evaluate ethanolic extract are cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), lipooxygenase (5-LOX), secretory phospholipase-A2 (sPLA2) inhibition assay whilst the in-vivo effect was measured by the ability of aqueous extracts to reduce paw edema induced by λ-carrageenan, in rats. Results The ethanolic extract inhibited the activities of 5-LOX and COX-1(p--.05) whilst the inhibitory effect on COX-2 was only moderate. A marked inhibition of 5-LOX was observed at 30?μg/ ml. The extract did not inhibit the activity of sPLA2. The ability of the ethanolic extracts of Polygonum minus to inhibit both 5-LOX and COX, prompted a study to evaluate the effects of using an aqueous extract of Polygonum minus (LineminusTM); as this would be more suitable for future clinical testing. The anti-inhibitory activity of the aqueous extract from this plant was evaluated using a rat model where inflammation was induced in the paws by injection of λ-carrageenan. The aqueous extracts from Polygonum minus administered at doses of 100 and 300?mg/kg body weight (b.w.), significantly (p--.01) reduced paw edema induced by λ-carrageenan in the experimental model, at 4?h compared to the vehicle control. Furthermore, administration of 100?mg/kg b.w. or 300?mg/kg b.w. completely reduced inflammation of the paw 4?h after injection. Conclusion These findings suggest that aqueous extract of Polygonum minus possesses potent anti-inflammatory activities.