Impact of Different Lignin Fractions on Saccharification Efficiency in Diverse Species of the Bioenergy Crop Miscanthus

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• 作者：Tim van der Weijde ; Andres F. Torres ; Oene Dolstra…
• 关键词：Klason lignin ; Acetyl bromide lignin ; Acid detergent lignin ; Saccharification efficiency ; Miscanthus ; Bioenergy
• 刊名：BioEnergy Research
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：9
• 期：1
• 页码：146-156
• 全文大小：748 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biomaterials
  Biochemical Engineering
  Bioorganic Chemistry
  
• 出版者：Springer New York
• ISSN：1939-1242

文摘

Lignin is a key factor limiting saccharification of lignocellulosic feedstocks. In this comparative study, various lignin methods—including acetyl bromide lignin (ABL), acid detergent lignin (ADL), Klason lignin (KL), and modified ADL and KL determination methods—were evaluated for their potential to assess saccharification efficiency. Six diverse accessions of the bioenergy crop miscanthus were used for this analysis, which included accessions of Miscanthus sinensis, Miscanthus sacchariflorus, and hybrid species. Accessions showed large variation in lignin content. Lignin estimates were different between methods, but (highly) correlated to each other (0.54 ≤ r ≤ 0.94). The strength of negative correlations to saccharification efficiency following either alkaline or dilute acid pretreatment differed between lignin estimates. The strongest and most consistent correlations (−0.48 ≤ r ≤ −0.85) were obtained with a modified Klason lignin method. This method is suitable for high throughput analysis and was the most effective in detecting differences in lignin content (p < 0.001) between accessions.