Size Effects on the Interaction of QDs with the Mitochondrial Membrane In Vitro

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• 作者：Lu Lai ; Ya-Ping Li ; Ping Mei ; Wu Chen ; Feng-Lei Jiang…
• 关键词：CdTe Quantum dots ; Mitochondria ; Model membranes
• 刊名：Journal of Membrane Biology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：249
• 期：6
• 页码：757-767
• 全文大小：2,774 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Human Physiology
  
• 出版者：Springer New York
• ISSN：1432-1424
• 卷排序：249

文摘

The mitochondrial toxicity induced by GSH-CdTe Quantum dots (QDs) of different sizes was investigated. The decreases in absorbance and transmission electron microscopy images show that QDs induce the swelling of mitochondria. Results of flow cytometry indicate that QDs cause a reduction of mitochondrial membrane potential (MMP). A remarkable increase in fluidity of protein regions of mitochondrial membrane is observed, whereas the lipid regions are not obviously affected. Cyclosporin A (CsA) effectively prevents the QD-induced mitochondrial swelling. On the basis of these results, it is proposed that QDs induce mitochondrial permeability transition (MPT). Moreover, with increasing QDs size, a pronounced MPT is observed. The difference between the membrane fluidity induced by QDs and Cadmium ion and the ineffective protective effects of EDTA suggests that the mitochondrial toxicity of QDs cannot be only attributed to the release of metal ion. The protective effects of HSA indicate that the interaction of QDs with pore-forming protein gives rise to the increase in membrane fluidity. This hypothesis is demonstrated by the interaction of QDs with model membranes and proteins using differential scanning calorimetry and isothermal titration microcalorimetry. In conclusion, as the size of QDs increases, the binding affinity of QDs with membrane protein increases, and therefore causes a pronounced mitochondrial damage.