Random Phage Display-Based Screening of Peptides that Bind to Botulinum Neurotoxin Binding Protein, Nontoxic Nonhemagglutinin

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• 作者：Yoshimasa Sagane (1)
  Keita Miyata (1) (2)
  Sayuri Kurihara (1)
  Tohru Yoneyama (3)
  Ken Inui (1) (2)
  Shin-Ichiro Miyashita (1)
  Shintaro Hayashi (1)
  Tomonori Suzuki (4)
  Koichi Niwa (1)
  Toshihiro Watanabe (1)
  
• 刊名：Current Microbiology
• 出版年：2013
• 出版时间：August 2013
• 年：2013
• 卷：67
• 期：2
• 页码：188-192
• 全文大小：488KB
• 参考文献：1. Li L, Singh BR (1999) Structure鈥揻unction relationship of clostridial neurotoxins. J Toxicol Toxin Rev 18:95鈥?12 CrossRef
  2. Montecucco C, Schiavo G (1994) Mechanism of action of tetanus and botulinum neurotoxins. Mol Microbiol 13:1鈥? CrossRef
  3. Miyata K, Yoneyama T, Suzuki T, Kouguchi H, Inui K, Niwa K, Watanabe T, Ohyama T (2009) Expression and stability of the nontoxic component of the botulinum toxin complex. Biochem Biophys Res Commun 384:126鈥?30 CrossRef
  4. Niwa K, Koyama K, Inoue S-I, Suzuki T, Hasegawa K, Watanabe T, Ikeda T, Ohyama T (2007) Role of nontoxic components of serotype D botulinum toxin complex in permeation through a Caco-2 cell monolayer, a model for intestinal epithelium. FEMS Immunol Med Microbiol 49:346鈥?52 CrossRef
  5. Ohishi I, Sakaguchi G (1980) Oral toxicities of / Clostridium botulinum type C and D toxins of different molecular sizes. Infect Immun 28:303鈥?09
  6. Gu S, Rumpel S, Zhou J, Stromeier J, Bigalke H, Perry K, Shoemaker CB, Rummel A, Jin R (2012) Botulinum neurotoxin is shielded by NTNHA in an interlocked complex. Science 335:977鈥?81 CrossRef
  7. Krumpe LRH, Atkinson AJ, Smythers GW, Kandel A, Schumacher KM, McMahon JB, Makowski L, Mori T (2006) T7 lytic phage-displayed peptide libraries exhibit less sequence bias than M13 filamentous phage-displayed peptide libraries. Proteomics 6:4210鈥?222 CrossRef
  8. Nishiyama K, Takakusagi Y, Kusayanagi T, Matsumoto Y, Habu S, Kuramochi K, Sugawara F, Sakaguchi K, Takahashi H, Natsugari H, Kobayashi S (2009) Identification of trimannoside-recognizing peptide sequences from a T7 phage display screen using a QCM device. Bioorg Med Chem 17:195鈥?02 CrossRef
  9. Takakusagi Y, Kuroiwa Y, Sugawara F, Sakaguchi K (2008) Identification of a methotrexate-binding peptide from a T7 phage display screen using a QCM device. Bioorg Med Chem 16:7410鈥?414 CrossRef
  10. Takakusagi Y, Takakusagi K, Kuramochi K, Kobayashi A, Sugawara F, Sakaguchi K (2007) Identification of C10 biotinylated camptothecin (CPT-10-B) binding peptides using T7 phage display screen on a QCM device. Bioorg Med Chem 15:7590鈥?598 CrossRef
  11. Hasegawa K, Watanabe T, Sato H, Sagane Y, Mutoh S, Suzuki T, Yamano A, Kouguchi H, Takeshi K, Kamaguchi A, Fujinaga Y, Oguma K, Ohyama T (2004) Characterization of toxin complex produced by a unique strain of / Clostridium botulinum serotype D 4947. Protein J 23:371鈥?78 CrossRef
  
• 作者单位：Yoshimasa Sagane (1)
  Keita Miyata (1) (2)
  Sayuri Kurihara (1)
  Tohru Yoneyama (3)
  Ken Inui (1) (2)
  Shin-Ichiro Miyashita (1)
  Shintaro Hayashi (1)
  Tomonori Suzuki (4)
  Koichi Niwa (1)
  Toshihiro Watanabe (1)
  
  1. Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri, 099-2493, Japan
  2. Japan Society for the Promotion of Science, 1-8 Chiyoda-ku, Tokyo, 102-8472, Japan
  3. Department of Advanced Transplantation and Tissue Engineering, Hirosaki University School of Medicine, Hirosaki, Aomori, 036-8562, Japan
  4. Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8558, Japan
  

文摘

Botulinum neurotoxin (BoNT) binds to nontoxic nonhemagglutinin (NTNHA) protein in a pH-dependent manner, and yields the protease-resistant BoNT/NTNHA complex. Here, we screened short peptides that bind to the serotype D NTNHA (NTNHA-D) using random phage display technique. NTNHA was fixed onto electrode of quartz crystal microbalance (QCM) apparatus, and then the phages displaying random heptapeptides were exposed to the NTNHA-D under the acidic condition. After rinsing with acidic buffer, the released phages under the alkaline condition were collected. The binding and release of the phage were monitored by the frequency shift on the QCM. As a result of the screening, 16 were selected as peptides that bind to NTNHA-D. The selected peptides do not share any conserved sequence, but tend to be rich in basic and/or hydrophobic amino acid. This would explain the binding manner of the BoNT to the NTNHA protein.