Differential Proteome Between Patient-Related and Non-related Environmental Isolates of Legionella pneumophila

详细信息    查看全文

• 作者：Sara Quero ; Marian García-Núñez ; Noemí Párraga-Niño…
• 刊名：Current Microbiology
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：74
• 期：3
• 页码：344-355
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Microbiology; Biotechnology;
• 出版者：Springer US
• ISSN：1432-0991
• 卷排序：74

文摘

Molecular epidemiologic studies of Legionella have shown different molecular types coexisting in the same environment, with only one having the ability to trigger an outbreak. We therefore studied the proteome of isolates of these different molecular types in search of the proteins responsible for infection. In this study, we performed a differential proteomic analysis between patient-related and non-patient-related environmental isolates using two-dimensional difference gel electrophoresis (2D-DIGE) combined with mass spectrometry. Sixty-three spots were observed as being different between the two groups; 31 spots were identified corresponding to 23 different proteins. Patient-related isolates overexpressed proteins associated with metabolism, with enzymes of the tricarboxylic acid cycle and the degradation pathways being the most abundant proteins identified. However, the largest group of non-patient-related proteins was associated with stress response. Furthermore, the MOMP protein was located in different spots depending on their patient-related or non-patient-related origin, suggesting different post-translational modifications. According to these results, different bacterial adaptation pathways are activated in stress conditions which influence their ability to produce infection.