Cytotoxicity testing of a polyurethane nanofiber membrane modified with chitosan/β-cyclodextrin/berberine suitable for wound dressing application: evaluation of biocompatibility
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- 作者：Monika Klempaiová ; Jana Dragúňová ; Peter Kabát ; Mária Hnátová…
- 关键词：Modified polyurethane nanofiber membrane ; Plasma treatment ; Biocompatibility ; 3T3 murine fibroblasts ; Human dermal fibroblasts
- 刊名：Cell and Tissue Banking
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：17
- 期：4
- 页码：665-675
- 全文大小：3,149 KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Cell Biology
Biomedicine
Transplant Surgery - 出版者：Springer Netherlands
- ISSN：1573-6814
- 卷排序：17
文摘
In this study we evaluated the biocompatibility of a modified polyurethane nanofiber membrane on a polypropylene spunbond substrate. This material was treated with plasma using diffuse coplanar surface barrier discharge, and subsequent modification was done by continuous spraying of a biologically active chitosan solution (CHIT) containing an inclusion complex of β-cyclodextrin (β-CD) encapsulating berberine (BRB). Biocompatibility was evaluated using several in vitro assays. Human dermal fibroblasts (HDFs) and 3T3 murine fibroblasts were used as biological models. The results of these assays showed that a polyurethane nanofiber membrane modified by CHIT/β-CD/BRB appears to be non-toxic and biocompatible; potentially, it could be used as a wound dressing after further testing.