Positive association of Apolipoprotein E4 polymorphism with recurrent pregnancy loss in Iranian patients
详细信息 查看全文
- 作者：Nooshin Asgari (1)
Mohammad Taghi Akbari (2) (3)
Shohre Zare (3) (4)
Gholamreza Babamohammadi (3) - 关键词：Apo E4 polymorphism ; Apolipoprotein E ; Recurrent pregnancy loss
- 刊名：Journal of Assisted Reproduction and Genetics
- 出版年：2013
- 出版时间：February 2013
- 年：2013
- 卷：30
- 期：2
- 页码：265-268
- 全文大小：131KB
- 参考文献：1. Stirrat GM. Recurrent miscarriage. Lancet. 1990;336:673-. CrossRef
2. Clifford K, Rai R, Regan L. Future pregnancy outcome in unexplained recurrent first trimester miscarriage. Hum Reprod. 1997;12:387-. doi:10.1093/humrep/12.2.387 . CrossRef
3. Toth B, Jeschke U, Rogenhofer N, Scholz C, Würfel W, Thaler CJ, Makrigiannakis A. Recurrent miscarriage: current concepts in diagnosis and treatment. J Reprod Immunol. 2010;85:25-2. doi:10.1016/j.jri.2009.12.006 . CrossRef
4. Suzumori N, Sugiura Ogasawara M. Genetic factors as a causes of miscarriage. Curr Med Chem. 2010;17:3431-. CrossRef
5. Zammiti W, Mtiraoui N, Khairi H, Gris JC, Almawi WY, Mahjoub T. Associations between tumor necrosis factor-alpha and lymphotoxin-alpha polymorphisms and idiopathic recurrent miscarriage. Reproduction. 2008;135:397-03. doi:10.1530/REP-07-0322 . CrossRef
6. Parveen F, Faridi RM, Das V, Tripathi G, Agrawal S. Genetic association of phase I and phase II detoxification genes with recurrent miscarriages among North Indian women. Mol Hum Reprod. 2010;16:207-4. doi:10.1093/molehr/gap096 . CrossRef
7. Pabinger I. Thrombophilia and its impact on pregnancy. Thromb Res. 2009;123:S16-1. doi:10.1016/S0049-3848(09)70128-8 . CrossRef
8. Stortoni P, Cecati M, Giannubilo SR, Sartini D, Turi A, Emanuelli M, Tranquilli AL. Placental thrombomodulin expression in recurrent miscarriage. Reprod Biol Endocrinol. 2010;8:1-. doi:10.1186/1477-7827-8-1 . CrossRef
9. Goodman C, Goodman CS, Hur J, Jeyendran RS, Coulam C. The association of apoprotein E polymorphisms with recurrent pregnancy loss. Am J Reprod Immunol. 2009;61:34-. doi:10.1016/j.fertnstert.2009.12.016 . CrossRef
10. Wernette-Hammond ME, Lauer SJ, Corsini A, Walker D, Taylor JM, Rall Jr SC. Glycosylation of human apolipoprotein E. The carbohydrate attachment site is threonine 194. J Biol Chem. 1989;15:9094-01.
11. Mahley RW. Apolipoprotein E: cholesterol transport protein with expanding role in cell biology. Science. 1988;240:622-0. doi:10.1126/science.3283935 . CrossRef
12. Nathan BP, Bellosta S, Sanan DA, Weisgraber KH, Mahley RW, Pitas RE. Differential effects on apolipoproteins E3 and E4 on neuronal growth in Vitro. Science. 1994;264:850-. doi:10.1126/science.8171342 . CrossRef
13. Mahley RW, Weisgraber KH, Huang Y. Apolipoprotein E: structure determines function, from atherosclerosis to Alzheimers disease to AIDS. J Lipid Res. 2009;50:S183-88. doi:10.1194/jlr.R800069-JLR200 . CrossRef
14. Zhang H, Wu LM, Wu J. Cross-talk between apolipoprotein E and cytokines. Mediators Inflamm. 2011;2011:949072. doi:10.1155/2011/949072 .
15. Williams PJ, Broughton Pipkin F. The genetics of pre-eclampsia and other hypertensive disorders of pregnancy. Best Pract Res Clin Obstet Gynaecol. 2011;25:405-7. doi:10.1016/j.bpobgyn.2011.02.007 . CrossRef
16. Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 1988;16:1215. CrossRef
17. Zuo L, van Dyck CH, Luo X, Kranzler HR, Yang BZ, Gelernter J. Variation at APOE and STH loci and Alzheimer’s disease. Behav Brain Funct. 2006;2:13-3. doi:10.1186/1744-9081-2-13 . CrossRef
18. Mahley RW, Rall Jr SC. Apolipoprotein E: far more than a lipid transport protein. Annu Rev Genomics Hum Genet. 2000;1:507-7. doi:10.1146/annurev.genom.1.1.507 . CrossRef
19. Amundsen T, Ueland PM, Waage A. Plasma homocysteine levels in patients with deep venous thrombosis. Arterioscler Thromb Vasc Biol. 1995;15:1321-. doi:10.1161/01.ATV.15.9.1321 . CrossRef
20. Ozornek H, Ergin E, Jeyendran RS, Ozay AT, Pillai D, Coulam C. Is Apolipoprotein E codon 112 polymorphisms associated with recurrent pregnancy loss? Am J Reprod Immunol. 2010;64:87-2. doi:10.1111/j.1600-0897.2010.00814.x .
21. Vassiliadis S, Ranella A, Papadimitriou L, Makrygiannakis A, Athanassakis I. Serum levels of pro and anti-inflammatory cytokines in non-pregnant women, during pregnancy, labour and abortion. Mediators Inflamm. 1998;7:69-2. CrossRef
22. Jofre-Monseny L, Loboda A, Wagner AE, Huebbe P, Boesch-Saadatmandi C, Jozkowicz A, Minihane AM, Dulak J, Rimbach G. Effects of apoE genotype on macrophage inflammation and heme oxygenase-1 expression. Biochem Biophys Res Commun. 2007;357:319-4. doi:10.1016/j.bbrc.2007.03.150 . CrossRef
23. Chaouat G, Menu E, de Smedt D, Khrihnan L, Hui L, Assal Meliani A, Martal J, Raghupathy R, Wegmann TG. The emerging role of IL-10 in pregnancy. Am J Reprod Immunol. 1996;35:325-. doi:10.1111/j.1600-0897.1996.tb00488.x . CrossRef
24. Tziakas DN, Chalikias GK, Antonoglou CO, Veletza S, Tentes IK, Kortsaris AX, Hatseras DI, Kaski JC. Apolipoprotein E genotype and circulating interleukin-10 levels in patients with stable and unstable coronary artery disease. J Am Coll Cardiol. 2006;48:2471-1. doi:10.1016/j.jacc.2006.08.032 . CrossRef
25. Yenicesu GI, Cetin M, Ozdemir O, Cetin A, Ozen F, Yenicesu C, Yildiz C, Kocak N. A Prospective Case–Control Study analyzes 12 thrombophilic gene mutations in turkish couples with recurrent pregnancy loss. Am J Reprod Immunol. 2010;63:126-6. doi:10.1111/j.1600-0897.2009.00770.x . CrossRef
26. Bianca S, Barrano B, Cutuli N, Indaco L, Cataliotti A, Milana G, Barone C, Ettore G. No association between apolipoprotein E polymorphisms and recurrent pregnancy loss. Fertil Steril. 2010;93:276. doi:10.1016/j.fertnstert.2009.07.971 . CrossRef
27. Goodman C, Coulam C, Jeyendran RS. Association of apolipoprotein E polymorphisms and recurrent pregnancy loss. Fertil Steril. 2010;93:e19. doi:10.1016/j.fertnstert.2009.12.016 . CrossRef - 作者单位：Nooshin Asgari (1)
Mohammad Taghi Akbari (2) (3)
Shohre Zare (3) (4)
Gholamreza Babamohammadi (3)
1. Department of biology, Sciences and Research Branch Islamic Azad University, Tehran, Iran
2. Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Jalal Al-Ahmad Express Way, Nasr Bridge, 14115331, Tehran, Iran
3. Tehran Medical Genetics Laboratory, Tehran, Iran
4. Department of Biology, Varamin Pishva Branch, Islamic Azad University, Varamin Pishva, Iran - ISSN：1573-7330
文摘
Problem Numerous lines of evidence implicate Apolipoprotein E (Apo E) in lipid metabolism during pregnancy. Hence, a role for its polymorphism has been envisaged in recurrent pregnancy loss (RPL) considering major structural and functional differences between different Apo E genotypes. Method of study A case control study of 81 women with two or more pregnancy losses that did not have any other known risk factors including anatomic anomalies of the reproductive system, infections, immunologic factors, hormonal imbalances, chromosomal abnormalities and environmental factors was carried out. The control group consisted of 81 women with at least two healthy children and no RPL in their reproductive history. DNA was extracted from the peripheral blood following written consent and Apo E genotyping was carried out by amplifying exon 4 of the gene and subjecting it to digestion by HhaI restriction enzyme. Results Genotyping was concluded by analyzing different fragment sizes produced, which resulted in finding significantly higher frequency of combined E3/E4 and E4/E4 genotypes in the patients (about 20?%) compared to the normal controls (2.4?%). The genotypes were confirmed by DNA sequencing. Conclusion Allelic frequency for E4 was 13.5?% in the patients and only 1?% in the non-RPL group. Our findings confirm and are in line with a number of similar studies carried out on other populations. Therefore, Apo E4 polymorphism seems to be contributing to the thrombophilic risk factors as a background to RPL.