TB trifusion antigen adsorbed on calcium phosphate nanoparticles stimulates strong cellular immunity in mice
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- 作者：Yousef Amini ; Bagher Moradi ; Mohsen Tafaghodi…
- 关键词：tuberculosis ; HspX ; Ppe44 ; EsxV ; calcium phosphate ; nanoparticle ; immune response
- 刊名：Biotechnology and Bioprocess Engineering
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：21
- 期：5
- 页码：653-658
- 全文大小：336 KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Biotechnology - 出版者：The Korean Society for Biotechnology and Bioengineering
- ISSN：1976-3816
- 卷排序：21
文摘
Among various vaccine candidates, subunit vaccines play an important role in immune protection against tuberculosis (TB). Calcium phosphate (CP) is considered as a strong inorganic adjuvant due to its great potential in increasing immune responses. The purpose of this study was to evaluate specific immune responses following the administration of trifusion-CP nanoparticles. The physiochemical properties of these nanoparticles, including morphology, particle size, zeta potential and adsorption rate, were measured in vitro. Subcutaneous immunization was performed three times on days 0, 14, and 28. Two weeks after the last administration, IFN-gamma, IL-4, and TGF-beta levels were measured by indirect enzyme linked immunosorbent assay (ELISA). The trifusion protein was successfully adsorbed onto calcium phosphate nanoparticles. The mean sizes of the resultant trifusion- CPN and CPN were 97.84 ± 12.08 and 67 ± 11.85 nm, respectively. CPN containing trifusion had stronger ability to induce IFN-gamma than the control groups. IL-4 and TGF-beta secretions in trifusion and trifusion-CPN groups were higher than those in the PBS group. However, there was no significant (p > 0.05) difference in IL-4 and TGFbeta concentrations between trifusion group and trifusion- CPN group. Therefore, calcium phosphate nanoparticles are good candidates for immunization against TB because antigen can be easily adsorbed onto CPN and strong cellular immune responses against CPN-antigen can be stimulated.