Plasmonic SERS biosensing nanochips for DNA detection

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• 作者：Hoan T. Ngo ; Hsin-Neng Wang ; Andrew M. Fales…
• 关键词：DNA detection ; Multiplex DNA detection ; DNA biosensor ; Surface ; enhanced Raman scattering ; Molecular sentinel ; on ; chip
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：408
• 期：7
• 页码：1773-1781
• 全文大小：1,008 KB
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• 作者单位：Hoan T. Ngo (1) (2)
  Hsin-Neng Wang (1) (2)
  Andrew M. Fales (1) (2)
  Tuan Vo-Dinh (1) (2) (3)
  
  1. Department of Biomedical Engineering, Duke University, Durham, NC, 27708, USA
  2. Fitzpatrick Institute for Photonics, Duke University, Durham, NC, 27708, USA
  3. Department of Chemistry, Duke University, Durham, NC, 27708, USA
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

The development of rapid, cost-effective DNA detection methods for molecular diagnostics at the point-of-care (POC) has been receiving increasing interest. This article reviews several DNA detection techniques based on plasmonic-active nanochip platforms developed in our laboratory over the last 5 years, including the molecular sentinel-on-chip (MSC), the multiplex MSC, and the inverse molecular sentinel-on-chip (iMS-on-Chip). DNA probes were used as the recognition elements, and surface-enhanced Raman scattering (SERS) was used as the signal detection method. Sensing mechanisms were based on hybridization of target sequences and DNA probes, resulting in a distance change between SERS reporters and the nanochip’s plasmonic-active surface. As the field intensity of the surface plasmon decays exponentially as a function of distance, the distance change in turn affects SERS signal intensity, thus indicating the presence and capture of the target sequences. Our techniques were single-step DNA detection techniques. Target sequences were detected by simple delivery of sample solutions onto DNA probe-functionalized nanochips and measuring the SERS signal after appropriate incubation times. Target sequence labeling or washing to remove unreacted components was not required, making the techniques simple, easy-to-use, and cost-effective. The usefulness of the nanochip platform-based techniques for medical diagnostics was illustrated by the detection of host genetic biomarkers for respiratory viral infection and of the dengue virus gene.