Improvement of monacolin K/citrinin production ratio in Monascus purpureus using UV mutagenesis

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• 作者：M. Kalaivani (1)
  A. Rajasekaran (2)
  
• 关键词：Monascus purpureus ; monacolin K ; UV mutation ; citrinin ; cross ; streak method
• 刊名：Nutrafoods
• 出版年：2014
• 出版时间：June 2014
• 年：2014
• 卷：13
• 期：2
• 页码：79-84
• 全文大小：261 KB
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• 作者单位：M. Kalaivani (1)
  A. Rajasekaran (2)
  
  1. Indian Pharmacopoeia Commission Ministry of Health and Family Welfare, Govt. of India, Sector-23, Raj Nagar, Ghaziabad, 201 002, Uttar Pradesh, India
  2. KMCH College of Pharmacy, Coimbatore, Tamil Nadu, India
  
• ISSN：2240-5291

文摘

Monascus purpureus MTCC 1090 is a fungus that produces monacolin K, gamma-aminobutyric acid (GABA) and citrinin under solid-state and submerged fermentation. The production of citrinin has a negative impact on its acceptance as a nutraceutical due to its neurotoxic, hepatotoxic and genotoxic effects. The purpose of the present study is to produce a M. purpureus mutant with zero concentration of citrinin production by random mutagenesis using ultra-violet (UV) irradiation. Mutants were screened on the basis of changes in their cultural characteristics and ratio of inhibition against Bacillus subtilis by the cross-streak method. Mutant M. purpureus 254 produced no citrinin as analysed by high-performance liquid chromatography and showed an increase in monacolin K production of about 74.32% in an optimised fermentation medium.