Putative Alginate Assimilation Process of the Marine Bacterium Saccharophagus degradans 2-40 Based on Quantitative Proteomic Analysis
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- 作者：Toshiyuki Takagi ; Hironobu Morisaka ; Shunsuke Aburaya…
- 关键词：Saccharophagus degradans ; Marine bacterium ; Quantitative proteomic analysis ; Alginate ; 4 ; Deoxy ; l ; erythro ; 5 ; hexoseulose uronic acid reductase
- 刊名：Marine Biotechnology
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：18
- 期：1
- 页码：15-23
- 全文大小：943 KB
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Hironobu Morisaka (1) (2)
Shunsuke Aburaya (1) (2)
Yohei Tatsukami (1) (2) (3)
Kouichi Kuroda (1) (2)
Mitsuyoshi Ueda (1) (2)
1. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto, 606-8502, Japan
2. JST, CREST, Kawaguchi, Saitama, 332-0012, Japan
3. Japan Society for the Promotion of Science, Sakyo, Kyoto, 606-8502, Japan - 刊物类别：Earth and Environmental Science
- 刊物主题：Earth sciences
Oceanography - 出版者：Springer New York
- ISSN：1436-2236
文摘
Quantitative proteomic analysis was conducted to assess the assimilation processes of Saccharophagus degradans cultured with glucose, pectin, and alginate as carbon sources. A liquid chromatography-tandem mass spectrometry approach was used, employing our unique, long monolithic silica capillary column. In an attempt to select candidate proteins that correlated to alginate assimilation, the production of 23 alginate-specific proteins was identified by statistical analyses of the quantitative proteomic data. Based on the analysis, we propose that S. degradans has an alginate-specific gene cluster for efficient alginate utilization. The alginate-specific proteins of S. degradans were comprised of alginate lyases, enzymes related to carbohydrate metabolism, membrane transporters, and transcription factors. Among them, the short-chain dehydrogenase/reductase Sde_3281 annotated in the alginate-specific cluster showed 4-deoxy-l-erythro-5-hexoseulose uronic acid reductase (DehR) activity. Furthermore, we found two different genes (Sde_3280 and Sde_0939) encoding 2-keto-3-deoxy-d-gluconic acid (KDG) kinases (KdgK) that metabolize the KDG derived from alginate and pectin in S. degradans. S. degradans used Sde_3280 to phosphorylate the KDG derived from alginate and Sde_0939 to phosphorylate the KDG derived from pectin. The distinct selection of KdgKs provides an important clue toward the elucidation of how S. degradans recognizes and processes polysaccharides. Keywords Saccharophagus degradans Marine bacterium Quantitative proteomic analysis Alginate 4-Deoxy-l-erythro-5-hexoseulose uronic acid reductase